Protective Effect of ATRA on Bleomycin Induced Lung Fibrosis in Rat
- *Corresponding Author:
- Ksouri Riadh
Laboratoire des Plantes Extrêmophiles
Centre de Biotechnologie à la Technopole de Borj Cédria (CBBC)
BP 901,2050 Hammam-lif, Tunisia
Received date: July 25, 2014; Accepted date: August 20, 2014; Published date: August 22, 2014
Citation: Raja SB, Najoua M, Anouar A, Oussama S, Saloua J, et al. (2014) Protective Effect of ATRA on Bleomycin Induced Lung Fibrosis in Rat. Med chem 4:611-616. doi:10.4172/2161-0444.1000202
Copyright: © 2014 Raja SB, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
All-trans-retinoic acid (ATRA), an active metabolite of vitamin A, is known to affect cell differentiation, proliferation, and development. Pulmonary fibrosis is one of the most common chronic interstitial lung diseases with high mortality rate after diagnosis and limited successful treatment. The aim of this study is to assess the effects of ATRA against bleomycin-induced pulmonary fibrosis in rats. Animals were intratracheally instillated with bleomycin and/or intraperitoneally administered with ATRA. On day 28, rats were sacrificed and histological changes in the lungs were evaluated. Moreover, malondialdehyde (MDA) content, superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) activities in serum and lung, were achieved. Results displayed that rat body weight decreased while fibrosis score and inflammatory index in lung tissue were significantly increased, after bleomycin instillation. Administration of bleomycin followed by ATRA reduced bleomycin–induced weight loss, decreased the lung index and the inflammatory indices. Histopathological examination confirmed the antifibrotic effect of ATRA which apparently attenuated the degree of pulmonary fibrosis. In addition, data showed that ATRA significantly increased SOD, CAT and GPx levels as compared to bleomycin group (G2) concomitant to the decrease of MDA content in lung homogenates. These findings indicate that ATRA treatment significantly attenuated the increased pulmonary damage induced by bleomycin.