alexa Protective Role of Gossypetin against Cyclophosphamide
ISSN: 2161-0444

Medicinal chemistry
Open Access

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Research Article

Protective Role of Gossypetin against Cyclophosphamide Toxicity in Human Lymphocyte Culture In vitro

Ustunsoy S1*, Akal ZU2 and Alpsoy L3

1Medical Faculty, Medical Biochemistry Department, Fatih University, 34500, Istanbul, Turkey

2Science and Art Faculty, Biology Department, Fatih University, 34500, Istanbul, Turkey

3Medical Faculty, Medical Biology Department, Fatih University, 34500, Istanbul, Turkey

*Corresponding Author:
Seyfettin Ustunsoy
Medical Faculty, Medical Biochemistry Department
Fatih University, 34500
Istanbul, Turkey
Tel: +90-212- 8663300/7021
E-mail: [email protected]

Received date: January 25, 2016; Accepted date: February 17, 2016; Published date: February 22, 2016

Citation: Ustunsoy S, Akal ZU, Alpsoy L (2016) Protective Role of Gossypetin against Cyclophosphamide Toxicity in Human Lymphocyte Culture In vitro. Med chem 6:088-092.doi:10.4172/2161-0444.1000330

Copyright: © 2016 Ustunsoy S, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

Abstract

Gossypetin is a flavonoid which has anti-mutagenic, anti-atherosclerotic, antioxidant, as well as cytoprotective and antimicrobial effects. The objective of this study was to investigate the cytoprotective role of gossypetin (GP) against cyclophosphamide (CP) toxicity in the human lymphocyte culture. Cytotoxic, necrotic and apoptotic effects of CP (1mM), GP (25, 50 and 100 μM) and combination of them (CP+GP) were studied by using MTT assay and Flow cytometry analysis. It was detected that CP significantly decreased cell viability rate via arresting cell cycle and increasing apoptosis/necroptosis. However, GP treatment reduced negative effects of CP at different concentrations. The most effective concentration of GP against CP toxicity was 25 μM. This concentration GP increased live cell number and cell viability, in addition decreased necrotic and late apoptotic cell quantity which were treated with CP. These results suggest that GP could attenuate the cytotoxic effects of CP and protect the healthy cells when it is used during chemotherapy.

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