alexa Protein Encapsulation into PLGA Nanoparticles by a Nove
ISSN: 2157-7439

Journal of Nanomedicine & Nanotechnology
Open Access

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Research Article

Protein Encapsulation into PLGA Nanoparticles by a Novel Phase Separation Method Using Non-Toxic Solvents

Amin Swed1, Thomas Cordonnier1, Fabrice Fleury2 and Frank Boury1*

1LUNAM University, UMR S-1066 INSERM, Micro-Nanomedécines Biomimétiques, 49933 Angers, France

2LUNAM University, Mechanism and Regulation of DNA repair-IMPACT, UFIP UMR CNRS 8626, 44322 Nantes, France

*Corresponding Author:
Frank Boury
LUNAM University
UMR S-1066 INSERM
Micro-Nanomedécines Biomimétiques
49933 Angers, France
Tel: 33-2-44-688528
Fax: 33-2-44-688546
E-mail: [email protected]

Received Date: September 24, 2014; Accepted Date: November 06, 2014; Published Date: November 15, 2014

Citation: Swed A, Cordonnier T, Fleury F, Boury F (2014) Protein Encapsulation into PLGA Nanoparticles by a Novel Phase Separation Method Using Non-Toxic Solvents. J Nanomed Nanotechnol 5:241. doi:10.4172/2157-7439.1000241

Copyright: © 2014 Swed A, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

Abstract

Nanoparticles of biocompatible and biodegradable polymers such as poly-lactic-co-glycolic acid (PLGA) are widely used as drug delivery systems for the administration of biomolecules like proteins. The purpose of this work is to validate a novel formulation method by a phase separation phenomenon using the non-toxic solvent glycofurol (GF) in order to encapsulate proteins into PLGA nanoparticles. Nanoprecipitates of a model protein (lysozyme) and a therapeutic protein (TGF-β1) were formed to ensure their stability upon subsequent encapsulation in PLGA nanoparticles. Good encapsulation efficiency was obtained with preservation of the structure integrity and protein bioactivity after encapsulation. PLGA nanoparticles were then characterized in terms of size, zeta potential and morphology. Moreover, residual solvent was quantified and in vitro release study of the encapsulated proteins was performed to demonstrate the efficacy of our encapsulation method in drug sustained release. Finally, cytocompatibility study of nanoparticles was performed. Thus, we developed an effective method based on the preliminary step of protein precipitation for the formulation of PLGA nanoparticles as protein carriers for biomedical applications.

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