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ISSN: 0974-276X

Journal of Proteomics & Bioinformatics
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Research Article

Protein Sample Treatment with Peptide Ligand Library: Coverage and Consistency

Lei Li1, ChengJun Sun1, Steve Freeby1, Dennis Yee1, Sylvie Kieffer-Jaquinod2, Luc Guerrier3, Egisto Boschetti3*, Lee Lomas1

1Bio-Rad Laboratories, Hercules, CA 94547, USA

2DSV/IRTSV Laboratoire EdyP, CEA, 38054 Grenoble, France

3Bio-Rad Laboratories, 92430 Marnes-la-Coquette, France

*Corresponding Author:
Dr. Egisto Boschetti
Bio-Rad Laboratories, 92430
Marnes-la-Coquette, France
E-mail: [email protected]

Received Date: November 08, 2009; Accepted Date: December 17, 2009; Published Date: December 18, 2009

Citation: Li L, Sun C, Freeby S, Yee D, Kieffer-Jaquinod S, et al. (2009) Protein Sample Treatment with Peptide Ligand Library: Coverage and Consistency. J Proteomics Bioinform 2: 485-494. doi: 10.4172/jpb.1000110

Copyright: © 2009 Li L, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Low-abundance protein detection in biological samples is one of the main challenges in proteomics investigations. One approach that makes the detection of rare species possible is the treatment of biological samples with solidphase combinatorial peptide ligand libraries. However, the use of combinations of ligands opens an uncertainty in that, since the diversity of the library is very large, aliquots of beads sampled from the library might not have fully comparable bead species each time. Reproducibility of experimental data with highly diverse libraries is therefore a main concern to address. This paper reports reproducibility data when aliquots of similar and different volumes of libraries are used at a certain sample to library ratio. Eluates from ligand libraries and other fractions are analyzed using various complementary methods such as two-dimensional gel electrophoresis, immunoassay and mass spectrometry. The collected data show a high level of consistency from sample to sample when processed with similar and variable bead volumes. Analytical determinations are all convergent with each other in considering the similarity of results. It is anticipated that this demonstration reinforces the possibility that differential proteomics studies, in particular for the discovery of protein targets of interest, can effectively be accomplished with combinatorial peptide libraries.

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