Proteoglycans Isolated from Bramble Shark Cartilage (Echinorhinus brucus) Inhibits Proliferation of MCF-7 Human Breast Cancer Cells by Inducing ApoptosisAjeeshkumar KK1*, Asha KK1, Vishnu KV1, Remyakumari KR1, Shyni K1, Reshma J2, Navaneethan R1, Linu B3 and Mathew S1
- *Corresponding Author:
- Ajeeshkumar KK
Biochemistry and Nutrition Division
Central Institute of Fisheries Technology
Cochin-29, Kerala, India
E-mail: [email protected]
Received date: June 22, 2017; Accepted date: July 03, 2017; Published date: July 24, 2017
Citation: Ajeeshkumar KK, Asha KK, Vishnu KV, Remyakumari KR, Shyni K, et al. (2017) Proteoglycans Isolated from Bramble Shark Cartilage (Echinorhinus brucus) Inhibits Proliferation of MCF-7 Human Breast Cancer Cells by Inducing Apoptosis. Biochem Pharmacol 6:226. doi: 10.4172/2167-0501.1000226
Copyright: © 2017 Ajeeshkumar KK, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Proteoglycans (PGs) were isolated from deep sea shark Echinorhinus brucus (bramble shark) cartilage and their anti-proliferative activity against MCF-7 cell lines was evaluated. To establish apoptosis involvement for cytotoxicity, the following assays were performed i.e., caspases 3 and 9 assay, double fluorescent staining and DNA laddering assay. PGs were isolated by DEAE-sephacel ion exchange chromatography and identification and purity confirmed by chemical composition such as total carbohydrate, total protein, hexosamine, uronic aicd, sulfated GAG, wavelength scan and modified AGE. To confirm apoptosis pathway expression of genes BAX, Bcl-2 and p53 were carried out. PGs (100 μg/mL) induced a significant rise in activity of caspase 3 and 9, major apoptotic markers. A significant rise in the expression was also observed for Bcl-2 and p53. Whereas, low BAX expression was observed for PGs (100 μg/mL) treated sample. Significant anti-proliferative effect was observed and the mechanism behind cell death was concluded to be apoptosis.