alexa Proteomic Alteration During Dormant Period of <em>Curcu
ISSN: 0974-276X

Journal of Proteomics & Bioinformatics
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

Proteomic Alteration During Dormant Period of Curcuma Longa Rhizomes

Daranee Chokchaichamnankit1, Pantipa Subhasitanont1, N. Monique Paricharttanakul1, Polkit Sangvanich2, Jisnuson Svasti1,3,4, Chantragan Srisomsap1,3*

1Laboratory of Biochemistry, Chulabhorn Research Institute, Bangkok 10210, Thailand

2Research Center for Bioorganic Chemistry, Department of Chemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand

3Chulabhorn Graduate Institute, Bangkok 10210, Thailand

4Department of Biochemistry and Center for Protein Structure and Function, Faculty of Science, Mahidol University, Bangkok 10400, Thailand

*Corresponding Author:
Dr. Chantragan Srisomsap
Laboratory of Biochemistry
Chulabhorn Research Institute
Vibhavadee Rangsit Road
Bangkok 10210, Thailand
Tel: (66-2)-5740622 to 33 ext. 3715
Fax: (66-2)-5740625 ext. 3716
E-mail:[email protected]

Received Date: July 10, 2009; Accepted Date: September 23, 2009; Published Date: September 25, 2009

Citation: Chokchaichamnankit D, Subhasitanont P, Paricharttanakul NM, Sangvanich P, Svasti J, et al. (2009) Proteomic Alterations During Dormant Period of Curcuma Longa Rhizomes. J Proteomics Bioinform 2: 382-395. doi: 10.4172/jpb.1000098

Copyright: © 2009 Chokchaichamnankit D, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

Abstract

Proteins involved during storage of Curcuma longa have not been investigated in detail. Proteins and nucleic acids are considered to be essential for the sprouting period. As numerous applications of proteomic approaches have been reported in many areas of biology, biochemistry and biomedicine, we chose to use proteomic technology to study the protein expression of Curcuma longa (Khaminchun) rhizome during dormancy and sprouting. Microscale solution-phase isoelectric focusing (Zoom) was employed to enrich the low abundance proteins in the pH range of 5.4-10 and improve the separation of those proteins in the acidic range from 3-5.4. Samples were drawn at seven-day intervals from harvest until the commencement of sprouting. The proteomic patterns of the storage period (0, 14, 21, 42, and 70 days) were studied and identified by LC/MS/MS in these two pH ranges. High levels of glyceraldehyde-3-phosphate dehydrogenase, a glycolytic enzyme, were present and in glycosylated and phosphorylated forms. Sporamin, the major storage protein of the tuberous roots of sweet potato, was highly expressed in the dormant period and lower expression was detected in the sprouting period. Moreover, the enzyme for catalyzing the synthesis of catechin, leucoanthocyanidin reductase, was found for the first time in the Curcuma longa rhizome. These results represent the first proteomic patterns during the storage period of Curcuma longa. For the rhizomes of Curcuma longa, visible sprouting was observed within 70 days after harvest.

Keywords

Share This Page

Additional Info

Loading
Loading Please wait..
 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords