Proteomic Alteration During Dormant Period of Curcuma Longa Rhizomes
- *Corresponding Author:
- Dr. Chantragan Srisomsap
Laboratory of Biochemistry
Chulabhorn Research Institute
Vibhavadee Rangsit Road
Bangkok 10210, Thailand
Tel: (66-2)-5740622 to 33 ext. 3715
Fax: (66-2)-5740625 ext. 3716
Received Date: July 10, 2009; Accepted Date: September 23, 2009; Published Date: September 25, 2009
Citation: Chokchaichamnankit D, Subhasitanont P, Paricharttanakul NM, Sangvanich P, Svasti J, et al. (2009) Proteomic Alterations During Dormant Period of Curcuma Longa Rhizomes. J Proteomics Bioinform 2: 382-395. doi: 10.4172/jpb.1000098
Copyright: © 2009 Chokchaichamnankit D, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Proteins involved during storage of Curcuma longa have not been investigated in detail. Proteins and nucleic acids are considered to be essential for the sprouting period. As numerous applications of proteomic approaches have been reported in many areas of biology, biochemistry and biomedicine, we chose to use proteomic technology to study the protein expression of Curcuma longa (Khaminchun) rhizome during dormancy and sprouting. Microscale solution-phase isoelectric focusing (Zoom) was employed to enrich the low abundance proteins in the pH range of 5.4-10 and improve the separation of those proteins in the acidic range from 3-5.4. Samples were drawn at seven-day intervals from harvest until the commencement of sprouting. The proteomic patterns of the storage period (0, 14, 21, 42, and 70 days) were studied and identified by LC/MS/MS in these two pH ranges. High levels of glyceraldehyde-3-phosphate dehydrogenase, a glycolytic enzyme, were present and in glycosylated and phosphorylated forms. Sporamin, the major storage protein of the tuberous roots of sweet potato, was highly expressed in the dormant period and lower expression was detected in the sprouting period. Moreover, the enzyme for catalyzing the synthesis of catechin, leucoanthocyanidin reductase, was found for the first time in the Curcuma longa rhizome. These results represent the first proteomic patterns during the storage period of Curcuma longa. For the rhizomes of Curcuma longa, visible sprouting was observed within 70 days after harvest.