Proteomic Analysis for the Purpose of Understanding the Mechanisms of Benzene and X-ray Induced Leukemia Using Human Bone Marrow Cells
- *Corresponding Author:
- Katsunori Sasaki
Kyoto University Graduate School of Engineering
Address: Kyoto-Daigaku Katsura 4
Nishikyo-ku, Kyoto-shi, Kyoto, 615-8540, Japan
Fax: 81-75- 383-3358
E-mail: [email protected]
Received Date: January 05, 2010; Accepted Date: February 22, 2010; Published Date:February 22, 2010
Citation: Sasaki K, Nishida Y, Adachi J, Okawa K, Nakayama A, et al. (2010) Proteomic Analysis for the Purpose of Understanding the Mechanisms of Benzene and X-ray Induced Leukemia Using Human Bone Marrow Cells. J Proteomics Bioinform 3: 066-073. doi: 10.4172/jpb.1000123
Copyright: © 2010 Sasaki K, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Benzene and the ionizing radiation are well known as leukemogens. There have been many studies on leukemia accumulated, but the mechanisms underlying the leukemogenicity are not fully understood. Since there are differences and similarities in leukemogenesis by benzene and radiation, comparative analysis could offer insight toward understanding basic leukemogenesis. In this study, we extracted proteins from CD34+ cells from human bone marrow, the target organ of leukemia, exposed to benzene metabolites (catechol and hydroquinone) or/and X-rays, and performed two dimensional gel image analysis. As a result, we identifi ed 8 proteins specifi c to benzene metabolites exposure, and 14 to X-ray irradiation. Notably, we found 2 proteins, protein SET and cofi lin-1, which showed changes in expression levels common to both benzene metabolites and X-ray exposure. These results suggest that the SET-PP2AJNK pathway might play a key role in the mechanisms of the leukemia.