Proteomic Analysis of Human Neuronal Cells Treated with the Gulf War Agent Pyridostigmine Bromide
Roskamp Institute, 2040 Whitfield Ave, Sarasota, FL, 34243
- *Corresponding Author:
- Dr. Laila Abdullah
Roskamp Institute, 2040
Whitfield Ave, Sarasota, FL, 34243
Tel : 941-752-2949
Fax : 941-752-2948
E-mail : [email protected]
Received Date: September 01, 2009; Accepted Date: October 13, 2009; Published Date: October 14, 2009
Citation: Abdullah L, Reed J, Kayihan G, Mathura V, Mouzon B, et al. (2009) Proteomic Analysis of Human Neuronal Cells Treated with the Gulf War Agent Pyridostigmine Bromide. J Proteomics Bioinform 2: 439-444. doi: 10.4172/jpb.1000103
Copyright: © 2009 Abdullah L, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Gulf War Illness (GWI) is characterized by a wide array of symptomology, which is possibly linked to the prophylactic treatment with pyridostigmine bromide (PB) against neurotoxins. It is now hypothesized that the pathological origin of this multi-symptom illness lies within the central nervous system and is caused by irregular activation of neuronal signaling pathways. To investigate this possibility, a proteomic-based approach was applied to characterize cellular responses of neuronal cells to PB exposure. Protein extracts from cultured neuroblastoma cells treated with 700nM PB for 10 days, as well as extracts from control cells were separated using two-dimensional gel electrophoresis. Twenty two differentially-expressed proteins were identified by MALDI-TOF mass spectrometry (MS). Ingenuity Pathways Analysis (IPA) software was then used to determine the biological functions and canonical pathways associated with the PB-responsive proteins.