alexa Proteomic Analysis of Ovarian Cancer Tumor Fluid is a Rich Source of Potential Biomarkers
ISSN: 0974-276X

Journal of Proteomics & Bioinformatics
Open Access

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Research Article

Proteomic Analysis of Ovarian Cancer Tumor Fluid is a Rich Source of Potential Biomarkers

Aline Poersch1, Lucas Oliveira Sousa2,3, Lewis Joel Greene2,3, Vitor Marcel Faça3,4* and Francisco Jose Candido dos Reis1

1Department of Gynecology and Obstetrics-FMRP, University of São Paulo, Av. Bandeirantes, 3900, 14040-900 Ribeirão Preto, SP, Brazil

2Department of Cell and Molecular Biology and Pathogenic Bioagents-FMRP-University of São Paulo, Av. Bandeirantes, 3900, 14040-900 Ribeirão Preto, SP, Brazil

3Center for Cell Based Therapy-Hemotherapy Center of Ribeirão Preto, Rua Tenente Catão Roxo, 2501, 14051-140 Ribeirão Preto, SP, Brazil

4Department of Biochemistry and Immunology-FMRP-University of São Paulo, Av. Bandeirantes, 3900, 14040-900 Ribeirão Preto, SP, Brazil

*Corresponding Author:
Vitor Marcel Faça
Center for Cell Based Therapy-Hemotherapy Center of Ribeirão Preto
Rua Tenente Catão Roxo, 2501
14051-140 Ribeirão Preto, SP, Brazil
Tel: (55)0162101-9300
E-mail: [email protected]

Received date: March 09, 2014; Accepted date: April 17, 2014; Published date: April 21, 2014

Citation: Poersch A, Sousa LO, Greene LJ, Faça VM, dos Reis FJC (2014) Proteomic Analysis of Ovarian Cancer Tumor Fluid is a Rich Source of Potential Biomarkers. J Proteomics Bioinform S5:004.doi:10.4172/jpb.S5-004

Copyright: © 2014 Poersch A, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Proteomic analysis of body fluids can be used to identify proteins associated with malignant transformation. We investigated the potential of hightroughput proteomics to profile the proteins in ovarian cancer tumor fluid. Tumor fluid samples were obtained from 10 women with high-grade ovarian serous carcinoma and from 10 women with benign serous cystadenoma. The samples were subjected to immunodepletion of albumin and IgG, followed by in-depth proteomic profiling by LC-MS/MS. A total of 1135 proteins were identified, 358 (32%) were detected in both malignant and benign pools, 505 (44%) only in the malignant pool, and 272 (24%) only in the benign pool. The proteomic profile of tumor fluid was similar to human plasma and the secretome of ovarian cancer cells. Proteins related to cellular metabolism were predominantly present in tumor samples. TIMP1 was determined by ELISA, confirming the proteomic data and its significantly higher level in individual malignant tumor fluids when compared with benign tumor fluids. In the present study, proteomic analysis of tumor fluid permited the identification of a large number of proteins expressed only in malignant tumors. Proteomic analysis of tumor fluid is a strategy in studies of biomarkers for ovarian cancer that can lead to significant advancements in the diagnosis and treatment of the disease.

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