Proteomic Analysis Of The "Side Population" (SP) Cells From Murine Bone Marrow
Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad 500 007, India
- *Corresponding Author:
- Dr. Gopal Pande
Centre for Cellular and Molecular Biology
Uppal Road, Hyderabad 500 007, India
Tel: +91 40 2719 2605
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Received Date: May 05, 2009; Accepted Date: September 29, 2009; Published Date: September 29, 2009
Citation: Satyavani R, Fatima A, Sundaram CS, Anabalagan C, Saritha CV, et al. (2009) Proteomic Analysis Of The “Side Population” (SP) Cells From Murine Bone Marrow. J Proteomics Bioinform 2: 398-407. doi: 10.4172/jpb.1000100
Copyright: © 2009 Satyavani R, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Side population (SP) cells are a specialized cell type present at very low numbers in murine bone marrow and other tissues. These cells are considered to be multipotent stem cells capable of differentiating into several cell lineages in vivo and ex vivo. In this report we have isolated SP cells from suspensions of bone marrow of Balb-c mice by using a fluorescence-activated cell sorter (FACS) and we have done the comparative analysis of proteins expressed by the total bone marrow (TBM) and SP cells, by using standard procedures of 2D gel electrophoresis (2DE) followed by MALDI-MS and/or MS/MS. Immunofluorescence analysis of the expression of cytoskeletal proteins actin and vimentin and a multi drug resistance (MDR) family protein ABCG2 were done to further validate the biochemical proteomic data. The classification of the identified proteins into their functional categories indicated that SP cells over express stress proteins, cytoskeletal proteins and enzymes of the glycolytic metabolism. These results could be significant in understanding the physiology of stem cells in the bone marrow.