alexa Proteomic Analysis Of The "Side Population" (SP) Cells
ISSN: 0974-276X

Journal of Proteomics & Bioinformatics
Open Access

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Research Article

Proteomic Analysis Of The "Side Population" (SP) Cells From Murine Bone Marrow

Ravipati Satyavani, Azra Fatima, Curam S. Sundaram, Charumathi Anabalagan, CV Saritha, G Srinivas, Aleem Ahmed Khan1, CM Habibullah1 and Gopal Pande*

Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad 500 007, India

1Deccan College of Medical Sciences, Kanchan Bagh, Hyderabad 500 0016, India

*Corresponding Author:
Dr. Gopal Pande
Centre for Cellular and Molecular Biology
Uppal Road, Hyderabad 500 007, India
Tel: +91 40 2719 2605
Fax : +91 40 2716 0591
E-mail: [email protected]

Received Date: May 05, 2009; Accepted Date: September 29, 2009; Published Date: September 29, 2009

Citation: Satyavani R, Fatima A, Sundaram CS, Anabalagan C, Saritha CV, et al. (2009) Proteomic Analysis Of The “Side Population” (SP) Cells From Murine Bone Marrow. J Proteomics Bioinform 2: 398-407. doi: 10.4172/jpb.1000100

Copyright: © 2009 Satyavani R, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

Abstract

Side population (SP) cells are a specialized cell type present at very low numbers in murine bone marrow and other tissues. These cells are considered to be multipotent stem cells capable of differentiating into several cell lineages in vivo and ex vivo. In this report we have isolated SP cells from suspensions of bone marrow of Balb-c mice by using a fluorescence-activated cell sorter (FACS) and we have done the comparative analysis of proteins expressed by the total bone marrow (TBM) and SP cells, by using standard procedures of 2D gel electrophoresis (2DE) followed by MALDI-MS and/or MS/MS. Immunofluorescence analysis of the expression of cytoskeletal proteins actin and vimentin and a multi drug resistance (MDR) family protein ABCG2 were done to further validate the biochemical proteomic data. The classification of the identified proteins into their functional categories indicated that SP cells over express stress proteins, cytoskeletal proteins and enzymes of the glycolytic metabolism. These results could be significant in understanding the physiology of stem cells in the bone marrow.

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