alexa Proteomic Analysis of Vascular Endothelial Cells-Effects of Laminar Shear Stress and High Glucose
ISSN: 0974-276X

Journal of Proteomics & Bioinformatics
Open Access

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Research Article

Proteomic Analysis of Vascular Endothelial Cells-Effects of Laminar Shear Stress and High Glucose

Xiao-Li Wang1*, Alex Fu2, Craig Spiro3, Hon-Chi Lee1

1Departments of Internal Medicine, Mayo Clinic, 200 First Street SW, Rochester, Minnesota 55905, USA

2Radiology, Mayo Clinic, 200 First Street SW, Rochester, Minnesota 55905, USA

3Center for Translational Science Activities, Mayo Clinic, 200 First Street SW, Rochester, Minnesota 55905,USA

*Corresponding Author:
Dr. Xiao-Li Wang, M.D., Ph.D.
Cardiovascular Division
Department of Internal Medicine, Mayo Clinic
200 First Street SW, Rochester, MN 55905
Whitfield Ave, Sarasota, FL, 34243
Tel : 507-255-9889
Fax : 507-538-6418
E-mail : [email protected]

Received Date: September 15, 2009; Accepted Date: October 22, 2009; Published Date: October 23, 2009

Citation: Wang XL, Fu A, Spiro C, Lee HC (2009) Proteomic Analysis of Vascular Endothelial Cells-Effects of Laminar Shear Stress and High Glucose. J Proteomics Bioinform 2: 445-454. doi: 10.4172/jpb.1000104

Copyright: © 2009 Wang XL, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

Abstract

This study directly measured the relative protein levels in bovine aortic endothelial cells (BAEC) that were cultured for two weeks in normal (5 mM, NG) or high (22 mM, HG) glucose and then were subjected to laminar shear stress at 0 or 15 dynes/cm2. Membrane preparations were labeled with one of the four isobaric tagging reagents (iTRAQ), followed by LC-MS/MS analysis. The results showed that HG and/or shear stress induced alterations in various membrane associated proteins involving many signaling pathways. While shear stress induced an increase in heat shock proteins and protein ubiquitination, which remained enhanced in HG, the effects of shear stress on the mechanosensing and protein phosphorylation pathways were altered by HG. These results were validated by Western blot analysis, suggesting that HG importantly modulates shear stress-mediated endothelial function.

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