Proteomic Analysis of Vascular Endothelial Cells-Effects of Laminar Shear Stress and High Glucose
- *Corresponding Author:
- Dr. Xiao-Li Wang, M.D., Ph.D.
Department of Internal Medicine, Mayo Clinic
200 First Street SW, Rochester, MN 55905
Whitfield Ave, Sarasota, FL, 34243
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Received Date: September 15, 2009; Accepted Date: October 22, 2009; Published Date: October 23, 2009
Citation: Wang XL, Fu A, Spiro C, Lee HC (2009) Proteomic Analysis of Vascular Endothelial Cells-Effects of Laminar Shear Stress and High Glucose. J Proteomics Bioinform 2: 445-454. doi: 10.4172/jpb.1000104
Copyright: © 2009 Wang XL, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
This study directly measured the relative protein levels in bovine aortic endothelial cells (BAEC) that were cultured for two weeks in normal (5 mM, NG) or high (22 mM, HG) glucose and then were subjected to laminar shear stress at 0 or 15 dynes/cm2. Membrane preparations were labeled with one of the four isobaric tagging reagents (iTRAQ), followed by LC-MS/MS analysis. The results showed that HG and/or shear stress induced alterations in various membrane associated proteins involving many signaling pathways. While shear stress induced an increase in heat shock proteins and protein ubiquitination, which remained enhanced in HG, the effects of shear stress on the mechanosensing and protein phosphorylation pathways were altered by HG. These results were validated by Western blot analysis, suggesting that HG importantly modulates shear stress-mediated endothelial function.