alexa Proteomic Changes in Pseudomonas aeruginosa Biofilm Cel
ISSN: 0974-276X

Journal of Proteomics & Bioinformatics
Open Access

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Research Article

Proteomic Changes in Pseudomonas aeruginosa Biofilm Cells after Adaptive Resistance Development

Idalina Machado1,3*, Laurent Coquet2, Maria Olívia Pereira1 and Thierry Jouenne2

1IBB – Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710–057 Braga, Portugal

2CNRS, UMR 6270, Laboratory “Polymères, Biopolymères, Surfaces”, PISSARO proteomic platform, Rouen University, France

3Department of Chemical Engineering, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, s/n, 4200-465 Porto, Portugal

*Corresponding Author:
Idalina Machado
Department of Chemical Engineering
Faculty of Engineering
University of Porto, Rua Dr. Roberto Frias
s/n, 4200-465 Porto, Portugal
Tel: 00351 22 508 3658
E-mail: [email protected]

Received Date: January 07, 2016; Accepted Date: March 02, 2016; Published Date: March 09, 2016

Citation: Machado I, Coquet L, Pereira MO, Jouenne T (2016) Proteomic Changes in Pseudomonas aeruginosa Biofilm Cells after Adaptive Resistance Development. J Proteomics Bioinform 9:058-062. doi:10.4172/jpb.1000390

Copyright: © 2016 Machado I, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

Abstract

This work investigates the outer membrane (OM) proteomic alteration of Pseudomonas aeruginosa biofilm cells after adaptation to benzalkonium chloride (BC) and ciprofloxacin (CIP). Biofilms were formed in 6-well plates for 24 h being after submitted to 324 mg/L BC and 6.0 mg/L CIP, during 12 days. Cells were harvested, the OM proteins extracted and patterns compared. P. aeruginosa adaptation altered the total amount of six proteins, about 10% of the discriminated proteins on two-dimensional gels. Exposure to both antimicrobials generated a common downregulation of three proteins, GroEL, the major capsid protein and the putative tail sheath protein. The type 4 fimbrial biogenesis outer membrane protein PilQ precursor was over-expressed only in biofilm cells submitted to BC, while the probable bacteriophage protein and the hypothetical protein PA0537 were overexpressed in CIP exposed biofilm cells. The alterations in OMPs expression might be involved in the biofilm bacteria high resistance to antimicrobial agents.

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