Purification and Biochemical Characterization of Xylanases from Bacillus Pumilus and their Potential for Hydrolysis of PolysaccharidesChundakkadu Asha Poorna*
Biotechnology Division, National Institute for Interdisciplinary Science and Technology (Formerly Regional Research Laboratory) (CSIR), Trivandrum- 695019, India
- *Corresponding Author:
- Chundakkadu Asha Poorna
Plant Molecular Biology Division
Rajiv Gandhi Center for Biotechnology
Government of India, Trivandrum-695 014, India
E-mail: [email protected]
Received Date: October 27, 2011; Accepted Date: December 13, 2011; Published Date: December 15, 2011
Citation: Poorna CA (2011) Purification and Biochemical Characterization of Xylanases from Bacillus Pumilus and their Potential for Hydrolysis of Polysaccharides. Ferment Technol 1:101. doi: 10.4172/2167-7972.1000101
Copyright: © 2011 Poorna CA. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Extracellular xylanases free of cellulase produced by the alkalophilic bacteria Bacillus pumilus was purified to homogeneity throughout the precipitation with (NH4) 2SO4 , Q-Sepharose chromatography and characterized. The purified xylanases were proteins, with molecular mass ~14 kDa (Xyl 1), ~ 35 kDa (Xyl 2) and ~ 60 kDa (Xyl 3) as determined by SDS-PAGE. The optimal temperature and pH for the action of the enzyme were at 50 o C and 7 respectively. They exhibited thermal stability over a range of 20 to 40 o C at pH-7 and has retained 85 % at 60 o C. The activity strongly inhibited by 10 mm of Hg2+ , SDS and Fe2+ . The xylanase exhibited Km and Vmax values were 4.0 mg/ ml, 5000 μmol/ min/ mg protein (Xyl 1) as well as 3.5 mg /ml, 3448 μmol/ min/ mg of protein (Xyl 2) for oatspelt xylan.