alexa Purification of A1PI from Human Plasma-An Improved Process to Achieve Therapeutic Grade Purity
ISSN: 2157-7064

Journal of Chromatography & Separation Techniques
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

Purification of A1PI from Human Plasma-An Improved Process to Achieve Therapeutic Grade Purity

Nuvula Ashok Kumar*, Korla Lakshmana Rao, Zinia Chakraborthy , Archana Giri and Komath Uma Devi

*Centre for Biotechnology, JNTUH, Hyderabad, India

*Corresponding Author:
Ashok Kumar N
Centre for Biotechnology
JNTUH, Hyderabad, Telangana, India
Tel: 91-986-669-6098
E-mail: [email protected]

Received date: May 21, 2015; Accepted date: June 04, 2015; Published date: June 10, 2015

Citation: Kumar NA, Rao KL, Chakraborthy Z, Giri A, Devi KU (2015) Purification of A1PI from Human Plasma-an Improved Process to Achieve Therapeutic Grade Purity. J Chromatogr Sep Tech 6:277. doi: 10.4172/2157-7064.1000277

Copyright: © 2015 Kumar NA, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

Abstract

Alpha-1-proteinase inhibitor (A1PI) also known as α-anti trypsin (AAT) is a member of serine protease inhibitors super family present in human plasma. A1PI is the only known inhibitor of elastase but also inhibits trypsin and chymotrypsin. A1PI is a molecule with lot of therapeutic importance and is widely used in the treatment of emphysema or chronic obstructive pulmonary disease (COPD). There are many methods detailed in scientific articles and patents to purify A1PI but the current research describes a simple three step chromatography procedure to fractionate A1PI directly from human plasma. The current purification process starts from human plasma unlike most of the existing methods where Cohn fraction IV-1 paste is the starting material. This method excludes the use of more methods like Cohn ethanol fractionation, precipitation or affinity capture. The A1PI purified by this scheme is characterized and found to be a single chain glycoprotein with a molecular weight of 52 KDa with increased purity. As the process is devoid of precipitation and affinity steps, this scheme is easily scalable, economical to adapt for manufacturing A1PI with good yields of 0.6-0.7 gm of A1PI/L of plasma.

Keywords

Share This Page

Additional Info

Loading
Loading Please wait..
 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

[email protected]

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

[email protected]

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001 Extn: 9042

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords