alexa Purification of Avian IgY with Trichloroacetic Acid (TCA) | OMICS International | Abstract
ISSN: 2157-7064

Journal of Chromatography & Separation Techniques
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Research Article

Purification of Avian IgY with Trichloroacetic Acid (TCA)

Hellen Asemota1, Suzette Curtello1, Angel Alberto Justiz Vaillant2*, Wayne Mohammed2, Sehlule Vuma2, Chalapathi Rao AV2, Arvind Kurhade2, Shivnarine Kissoon2, Monica Smikle3, Brian Wisdom4 and Geeta Kurhade5

1Department of Basic Medical Sciences, The University of the West Indies, Mona, Jamaica

2Pathology and Microbiology Unit, Department of Para-Clinical Sciences, The University of the West Indies, St. Augustine, Trinidad and Tobago, WI

3Department of Microbiology, The University Hospital of West Indies Mona, Jamaica

4School of Biology and Biochemistry, Medical Biology Centre, The Queen’s University of Belfast, Belfast BT9 7BL, UK

5Physiology Section, Department of Pre-clinical Sciences, The University of the West Indies, St Augustine, Trinidad and Tobago, WI

*Corresponding Author:
Angel Alberto Justiz Vaillant
Pathology and Microbiology Unit
Department of Para-Clinical Sciences
The University of the West Indies
St. Augustine, Trinidad and Tobago, WI, USA
E-mail: [email protected]

Received Date: August 29, 2013; Accepted Date: October 18, 2013; Published Date: October 21, 2013

Citation: Asemota H, Curtello S, Vaillant AAJ, Mohammed W, Vuma S, et al. (2013) Purification of Avian IgY with Trichloroacetic Acid (TCA). J Chromatograph Separat Techniq 4:205. doi:10.4172/2157-7064.1000205

Copyright: © 2013 Asemota H, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

By using trichloroacetic acid (TCA) precipitation to separate egg yolk proteins, mainly IgY was demonstrated that this procedure yield twice more IgY that its counterpart polyethylene glycol (PEG). An ELISA demonstrated that it does not affect the functionality of the IgY molecule. The TCA precipitation procedure provides an efficient and rapid way to purify IgY antibody. It can be used for large scale production of high quality IgY.

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