alexa Purification of Immunoglobulin Y (IgY) from the Ostrich
ISSN: 2157-7064

Journal of Chromatography & Separation Techniques
Open Access

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Research Article

Purification of Immunoglobulin Y (IgY) from the Ostrich (Struthio camelus) by Staphylococcal Protein A (SpA) Affinity Chromatography

Angel Alberto Justiz Vaillant1, Patrick Eberechi Akpaka1*, Norman McFarlane-Anderson2, Monica P Smikle3 and Brian Wisdom4

1Department of Para-Clinical Sciences, Faculty of Medical Sciences, The University of the West Indies, St. Augustine, Trinidad & Tobago

2Departments of Basic Medical Sciences, Faculty of Medical Sciences, University of the West Indies, Mona, Kingston, Jamaica

3Department of Microbiology, Faculty of Medical Sciences, University of the West Indies, Mona, Kingston, Jamaica

4School of Biology and Biochemistry, Medical Biology Centre, The Queen’s University of Belfast, Belfast BT9 7BL

*Corresponding Author:
Patrick Eberechi Akpaka
Room 26, Block 5; Pathology/ Microbiology Unit
Eric Williams Medical Sciences Complex
Department of Para - Clinical Sciences
The University of the West Indies
St. Augustine Trinidad & Tobago
Tel:
868-645-2640; ext 2332/2322; 736-0440
Fax: 868-663-3797
E-mail: [email protected], [email protected]
Web: www.sta.uwi.edu

Received date: April 11, 2012; Accepted date: June 15, 2012; Published date: June 17, 2012

Citation: Justiz Vaillant AA, Akpaka PE, McFarlane-Anderson N, Smikle MP, Wisdom B(2012) Purification of Immunoglobulin Y (IgY) from the Ostrich (Struthio camelus) by Staphylococcal Protein a (Spa) Affinity Chromatography. J Chromat Separation Techniq 3:127. doi:10.4172/2157-7064.1000127

Copyright: © 2012 Justiz Vaillant AA, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

Abstract

Immunoglobulin Y (IgY) is the major protein present in the avian egg yolk. This antibody fulfils important functions in the protection of Ostrich birds against infections. The aim of this study was to demonstrate the binding capacity of Staphylococcal proteins A (SpA) to Ostrich IgY and assess purification of the IgY by SpA affinity chromatography. Chloroform polyethylene glycol (Polson), affinity chromatography, Enzyme-Linked Immunosorbent Assay (ELISA)and Western blotting methods were used in the process. Results obtained revealed that Ostrich IgY has heavy chain of 70 kDa and light chain of 30 kDa confirming results by Western blot. In addition livetins (egg yolk proteins)were shown in the protein electrophoresis that preceded the Western blot. The binding capacity between SpA and Ostrich IgY is important because SpA can be used as a reagent in immunoassays for antibody detection against microbial agents that usually infect livestocks. This is the first time the use of SpA for purification of Ostrich IgY is being reported in literature.

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