QRAR Models for Diuretics using mixed Micellar Liquid Chromatography
- *Corresponding Author:
- Dr. Li-Ming Ye
West China School of Pharmacy
Chengdu, 610041, China
Tel: +86 028 85502305
Fax: +86 02885503872
E-mail: [email protected]
Received Date: July 05, 2011; Accepted Date: August 25, 2011; Published Date: August 27, 2011
Citation: Wu L, Chen C, Sun C, Ye L (2011) QRAR Models for Diuretics using mixed Micellar Liquid Chromatography. J Bioequiv Availab 3: 169-173. doi: 10.4172/jbb.1000079
Copyright: © 2011 Wu L, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
In this article, the capability of traditional biopartitioning micellar chromatography (BMC), using the pure Brij35 solution and the mixed micellar system of Brij35/SDS (85:15) as mobile phase respectively, under adequate experimental conditions, to describe and estimate bioactivities of diuretics, has been focused. The BMCBrij35/SDS-QRAR models can simulate the resting membrane potential and the conformation of the long hydrophilic polyoxyethylene chains remains unchanged. The predictive and interpretative ability of the chromatographic models was evaluated in terms of cross-validated data (RMSEC, RMSECV and RMSECVi). The obtained BMCBrij35/SDS-QRAR were compared with the traditional BMCBrij35-QRAR models, and better statistically models were obtained using Brij35-SDS retention data.