Quantification of Allethrin using HPTLC from Annona Squamosa (Custard Apple)
- *Corresponding Author:
- Shardul kulkarni
Department of Chemistry
Guru Nanak Khalsa College
Matunga, Mumbai-400019, India
E-mail: [email protected]
Received date: December 03, 2012; Accepted date: December 20, 2012; Published date: December 24, 2012
Citation: Kulkarni S, Tekale PC, Lanjewar R, Joshi S, Chitalkar K, et al. (2012) Quantification of Allethrin using HPTLC from Annona Squamosa (Custard Apple). J Chromat Separation Techniq 3:157. doi:10.4172/2157-7064.1000157
Copyright: © 2012 Kulkarni S, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Annona squamosa (Custard Apple) is delicious fruit and it is traditionally used for curing a variety of ailments such as Ulser, dysentery, Antiovulatory, hair problems, burning sensation, hysteria (fearful state of mind) and fainting spells. There are no reports of quantification of Allthrine from this plant. Hence a HPTLC densitometric method has been developed and validated for quantification of this marker compound. Allethrine was quantified from methanolic extract using the solvent system of Petroleum ether: Diethyl ether (7: 3, v/v). The method was validated using ICH guidelines in terms of precision, repeatability and accuracy. Linearity range for Allethrine was 40–320 ng/spot and its content was 0.01046% w/w in peel and 0.01042% w/w in seed. This simple, precise and accurate method gave good resolution from other constituents of extract. It can be adopted for routine quality control of herbal material and formulations containing Annona squamosa (Custard Apple).