alexa Quantification of Antifungal Drug Voriconazole in Serum and Plasma by HPLC-UV
ISSN: 2157-7609

Journal of Drug Metabolism & Toxicology
Open Access

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Research Article

Quantification of Antifungal Drug Voriconazole in Serum and Plasma by HPLC-UV

Peter H Tang*

Divisions of Pathology and Laboratory Medicine, Departments of Pathology and Pediatrics, Cincinnati Children’s Hospital Medical Center, University of Cincinnati College of Medicine, 3333 Burnet Avenue, Cincinnati, Ohio 45229-3039, USA

*Corresponding Author:
Peter H Tang, PhD
Divisions of Pathology and Laboratory Medicine
Departments of Pathology and Pediatrics
Cincinnati Children’s Hospital Medical Center
University of Cincinnati College of Medicine
MLC 1010, 3333 Burnet Ave
Cincinnati, OH 45229, USA
Tel: (513) 636-7328
Fax: (513) 636-8850
E-mail: [email protected]

Received date: February 05, 2013; Accepted date: March 18, 2013; Published date: March 20, 2013

Citation: Tang PH (2013) Quantification of Antifungal Drug Voriconazole in Serum and Plasma by HPLC-UV. J Drug Metab Toxicol 4:144. doi: 10.4172/2157-7609.1000144

Copyright: © 2013 Tang PH. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

A simple, sensitive and rapid high-performance liquid chromatographic (HPLC) method for the determination of voriconazole in human serum or plasma was developed. Voriconazole and internal standard clonazepam were extracted from plasma or serum with methanol and analyzed on a Microsorb-MV C18 column with ultraviolet (UV) detection set at wavelengths of 256 and 310 nm, respectively. The calibration curve was linear through the range of 0.1-10 mg/L using a 0.1 mL sample volume. The within-run and between-run precisions were all less than 6%. Accuracies ranged from 97 to 106%. Absolute recovery was 96.4 ± 1.3% for voriconazole. The method has been applied to monitor voriconazole use in order to ascertain clinical efficacy and minimize toxic effects.

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