alexa Quantification of Buprenorphine, Norbuprenorphine and 6-Monoacetylmorphine in Urine by Liquid Chromatography-Tandem Mass Spectrometry
ISSN: 2157-7064

Journal of Chromatography & Separation Techniques
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

Quantification of Buprenorphine, Norbuprenorphine and 6-Monoacetylmorphine in Urine by Liquid Chromatography-Tandem Mass Spectrometry

Chao Yuan, Katherine Lembright, Courtney Heideloff and Sihe Wang*

Department of Clinical Pathology, Cleveland Clinic, Cleveland, USA

*Corresponding Author:
Sihe Wang
LL3-3, 9500 Euclid Ave
Cleveland, OH 44195
Tel: 216-445-2634
Fax: 216-445-0212
E-mail: [email protected]

Received Date: January 23, 2013; Accepted Date: March 23, 2013; Published Date: March 26, 2013

Citation: Yuan C, Lembright K, Heideloff C, Wang S (2013) Quantification of Buprenorphine, Norbuprenorphine and 6-Monoacetylmorphine in Urine by Liquid Chromatography-Tandem Mass Spectrometry. J Chromat Separation Techniq 4:174. doi: 10.4172/2157-7064.1000174

Copyright: © 2013 Yuan C, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

Abstract

Monitoring pain management medications and illicit drugs in urine is commonly used to assess patient compliance. Previously, we developed a liquid chromatography tandem mass spectrometry (LC-MS/MS) method to measure 19 analytes important for pain management. In the current report, we validated this method for two additional drugs, buprenorphine and heroin. For buprenorphine, we quantified both the parent drug and its major metabolite, norbuprenorphine. For heroin, we monitored its unique metabolite, 6-monoacetylmorphine (6-MAM). Urine samples were subjected to enzymatic hydrolysis prior to turbulent flow online extraction and LC-MS/MS analysis. No matrix effect or interference was found. Lower limits of quantifications were 9.7, 9.6, and 4.9 ng/mL for buprenorphine, norbuprenorphine and 6-MAM, respectively. Within the linear range, analytical recovery was 80.5-113.0% for all analytes. Intra-assay and total coefficient of variations were between 0.2% and 10.3%. This method demonstrated consistent patient results (n=40) with the independent LC-MS/MS methods offered by two other laboratories. Percentage of glucuronide conjugation of 6-MAM varied from 0 to 45% in 8 patient urine samples positive for 6-MAM. In conclusion, we have successfully expanded current pain management panel to include buprenorphine and heroin with high sensitivity, specificity, and precision.

Keywords

Share This Page

Additional Info

Loading
Loading Please wait..
 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

[email protected]

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

[email protected]

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001 Extn: 9042

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords