alexa Quantification of Pesticide Residues in Vegetables by Different Chromatographic Techniques
ISSN: 2157-7064

Journal of Chromatography & Separation Techniques
Open Access

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Research Article

Quantification of Pesticide Residues in Vegetables by Different Chromatographic Techniques

Anam Munawar* and Syed Waqas Hameed

Punjab Forensic Science Agency, Pakistan

*Corresponding Author:
Anam Munawar
Punjab Forensic Science Agency, Pakistan
Tel: 03336476622
E-mail: [email protected]

Received Date: August 07, 2013; Accepted Date: September 07, 2013; Published Date: September 10, 2013

Citation: Munawar A, Hameed SW (2013) Quantification of Pesticide Residues in Vegetables by Different Chromatographic Techniques. J Chromatograph Separat Techniq 4:200. doi:10.4172/2157-7064.1000200

Copyright: © 2013 Munawar A, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.



Cypermethrin, chlorpyrifos and imidacloprid residues were determined in different vegetables. During 2013, samples were collected from different six major vegetable markets from Lahore. Ethyl acetate was used for extraction, whattman fluted filter and charcoal was used for cleaning procedure. High performance thin layer chromatography was used for detection, as well as quantification of pesticides. Confirmation of results was done by using gas chromatography/mass spectrophotometer. From results, it was concluded that different vegetables contain different concentration of pesticides means adsorption rate is different for each pesticide and vegetable. 79% samples were contaminated with cypermethrin, 70% from imidacloprid and 65% samples were contaminated from chlorpyrifos. Pumpkin, okra, egg plant, cucumber spinach and cabbage were contaminated with pesticides. Peeling of vegetables resulted in less concentration of pesticide.


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