Quantification of Ticlopidine in Human Plasma Using Protein Precipitation and Liquid Chromatography Coupled with Tandem Mass SpectrometrySoJeong Yi1, Hyun-Suk Shin2, Seo-Hyun Yoon1, Kyung-Sang Yu1, In-Jin Jang1, Sang-Goo Shin1and Joo-Youn Cho1,*
- *Corresponding Author:
- Joo-Youn Cho, PhD
Department of Clinical Pharmacology & Therapeutics
Seoul National University College of Medicine and Hospital
101 Daehangno, Jongno-gu, Seoul, Republic of Korea, 110-744
E-mail: [email protected]
Received Date: January 19, 2011; Accepted Date: February 24, 2011; Published Date: March 07, 2011
Citation: Yi SJ, Shin HS, Yoon SH, Yu KS, Jang IJ, et al. (2011) Quantification of Ticlopidine in Human Plasma Using Protein Precipitation and Liquid Chromatography Coupled with Tandem Mass Spectrometry. J Bioanal Biomed 3: 059-063. doi: 10.4172/1948-593X.1000044
Copyright: © 2011 Yi SJ, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
A simple and rapid method has been developed and validated for determination of ticlopidine in human plasma using protein precipitation and liquid chromatography coupled with tandem mass spectrometry (LC/MS/MS). Ticlopidine was extracted from 20-μL aliquots of human plasma by one-step protein precipitation with 980 μL of acetonitrile containing 10 ng/mL clopidogrel as an internal standard (IS). Chromatographic separation was performed on a reverse-phase Gemini C 18 column (50 mm x 2.0 mm, 5 μm) with an isocratic mobile phase (acetonitrile: 1 mM ammonium acetate in water = 75:25, v/v). Ticlopidine and IS were detected and quantified by tandem mass spectrometry with positive electrospray ionization using multiple reaction monitoring of the transition m/z 264.04 to m/z 154.20 for ticlopidine and m/z 322.40 to m/z 212.20 for IS. This method was linear over the concentrations ranging from 2 to 2000 ng/mL. The accuracy in the inter-batch assay was 92.4-95.6% and the precision was within 6.4% coefficient of variation. The validated method was successfully applied to the human pharmacokinetic study of ticlopidine.