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Quantification of Ticlopidine in Human Plasma Using Protein Precipitation and Liquid Chromatography Coupled with Tandem Mass Spectrometry | OMICS International | Abstract
ISSN: 1948-593X

Journal of Bioanalysis & Biomedicine
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Research Article

Quantification of Ticlopidine in Human Plasma Using Protein Precipitation and Liquid Chromatography Coupled with Tandem Mass Spectrometry

SoJeong Yi1, Hyun-Suk Shin2, Seo-Hyun Yoon1, Kyung-Sang Yu1, In-Jin Jang1, Sang-Goo Shin1and Joo-Youn Cho1,*

1Department of Clinical Pharmacology & Therapeutics, Seoul National University College of Medicine and Hospital, Seoul, Republic of Korea

2Analytical Chemistry Laboratory, Clinical Research Institute, Seoul National University Hospital, Seoul, Republic of Korea

*Corresponding Author:
Joo-Youn Cho, PhD
Department of Clinical Pharmacology & Therapeutics
Seoul National University College of Medicine and Hospital
101 Daehangno, Jongno-gu, Seoul, Republic of Korea, 110-744
Tel: +82-2-740-8286
Fax: +82-2-742-9252
E-mail: [email protected]

Received Date: January 19, 2011; Accepted Date: February 24, 2011; Published Date: March 07, 2011

Citation: Yi SJ, Shin HS, Yoon SH, Yu KS, Jang IJ, et al. (2011) Quantification of Ticlopidine in Human Plasma Using Protein Precipitation and Liquid Chromatography Coupled with Tandem Mass Spectrometry. J Bioanal Biomed 3: 059-063. doi: 10.4172/1948-593X.1000044

Copyright: © 2011 Yi SJ, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

A simple and rapid method has been developed and validated for determination of ticlopidine in human plasma using protein precipitation and liquid chromatography coupled with tandem mass spectrometry (LC/MS/MS). Ticlopidine was extracted from 20-μL aliquots of human plasma by one-step protein precipitation with 980 μL of acetonitrile containing 10 ng/mL clopidogrel as an internal standard (IS). Chromatographic separation was performed on a reverse-phase Gemini C 18 column (50 mm x 2.0 mm, 5 μm) with an isocratic mobile phase (acetonitrile: 1 mM ammonium acetate in water = 75:25, v/v). Ticlopidine and IS were detected and quantified by tandem mass spectrometry with positive electrospray ionization using multiple reaction monitoring of the transition m/z 264.04 to m/z 154.20 for ticlopidine and m/z 322.40 to m/z 212.20 for IS. This method was linear over the concentrations ranging from 2 to 2000 ng/mL. The accuracy in the inter-batch assay was 92.4-95.6% and the precision was within 6.4% coefficient of variation. The validated method was successfully applied to the human pharmacokinetic study of ticlopidine.

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