alexa Quantitative Analysis of 25-OH Vitamin D Using Supported Liquid Extraction and Liquid Chromatography - Mass Spectrometry | OMICS International | Abstract
ISSN: 2157-7064

Journal of Chromatography & Separation Techniques
Open Access

Like us on:

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

Quantitative Analysis of 25-OH Vitamin D Using Supported Liquid Extraction and Liquid Chromatography - Mass Spectrometry

Eduard Rogatsky*, Shaynah Browne, Min Cai, Harsha Jayatillake and Daniel Stein

Biomarker Analytical Resource Core, Einstein-Montefiore Institute for Clinical and Translational Research. Albert Einstein College of Medicine, USA

*Corresponding Author:
Eduard Rogatsky
Biomarker Analytical Resource Core
Einstein-Montefiore Institute for Clinical and Translational Research
Albert Einstein College of Medicine, USA
Tel: 718-430-3391
E-mail: [email protected]

Received date: March 27, 2014; Accepted date: May 20, 2014; Published date: May 25, 2014

Citation: Rogatsky E, Browne S, Cai M, Jayatillake H, Stein D (2014) Quantitative Analysis of 25-OH Vitamin D Using Supported Liquid Extraction and Liquid Chromatography - Mass Spectrometry. J Chromatograph Separat Techniq 5:224. doi:10.4172/2157-7064.1000224

Copyright: © 2014 Rogatsky E, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


We report a low sample volume LC/MS method for 25OH Vitamin D analysis. The method requires only 40 μl serum, is fully automatable, fast and sensitive. The method was successfully implemented for clinical analysis of infants for vitamin D deficiency. The 6 min method has a sensitivity range from 2 to 100 ng/ml. A 3 min method was validated from 5 to 100 ng/ml range. Fast analysis, low sample volume and high sensitivity were achieved by combination of supported liquid extraction sample preparation, UHPLC chromatography and fused-core chromatographic column for separation, and highly sensitive mass spectrometry for detection. We emphasize the importance of sample preparation quality for rugged LC/MS analysis. Using SLE-based sample preparation we successfully used only 40 μl of serum while achieving a LLOQ of 2 ng/ml. We found from assayed samples, that 3-12 month old infants were not Vitamin D deficient, compared to adults. The average level of 25(OH)D3 in infants was 57.3 compared to 38.0 ng/ml for adults in a Bronx, NY patient population.


Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2018-19
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

bornova escort

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals


[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

gen[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

© 2008- 2018 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version