Quantitative Analysis of L-Abrine and Ricinine Spiked into Selected Food Matrices by Liquid Chromatography-Tandem Mass Spectrometry
Shannon M Black*, Sadia Muneem, Deborah Miller-Tuck and Prince A Kassim
Maryland Department of Health and Mental Hygiene, 201 West Preston Street, Baltimore, MD 21201 United States
- *Corresponding Author:
- Shannon M Black
Maryland Department of Health and Mental Hygien
201 West Preston Street, Baltimore
MD 21201, United States
E-mail: [email protected]
Received date: January 01, 2015; Accepted date: February 17, 2015; Published date: February 20, 2015
Citation: Black SM, Muneem S, Miller-Tuck D, Kassim PA (2015) Quantitative Analysis of L-Abrine and Ricinine Spiked into Selected Food Matrices by Liquid Chromatography-Tandem Mass Spectrometry. J Chromatogr Sep Tech 6:265. doi:10.4172/2157-7064.1000265
Copyright: © 2015 Black SM, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abrin and ricin are highly toxic and lethal proteins which have the potential to be used as bioterrorism agents.Labrine and ricinine, molecular biomarkers for abrin and ricin, serve as useful biomarkers in assessing exposure and contamination.In this study, we developed a method for the quantitation of L-abrine and ricinine spiked into four food types: ground beef, chicken breast, hot dogs, and Eggbeaters®.This method involved sample homogenization, followed by polymeric reversed solid phase extraction, evaporation, and reconstitution.Determination and quantitation of L-abrine and ricinine was achieved using LC tandem mass spectrometry utilizing positive electrospray ionization. Quantitation was based upon fragmentation of m/z219→132 for L-abrine and m/z 165→138 for ricinine.The limit of detection achieved was 0.1 μg/L while the limit of quantitation was 0.50 μg/L for L-abrine and 0.30 μg/L for ricinine.A 7-point standard calibration curve showed good linearity for both analytes (r2 = 0.990) with a CV of <10% for replicates.Spiked fortified food matrices at 5 μg/L, 25 μg/L, and 50 μg/L yielded recoveries ranging from 93 – 119%. Validation results showed this method to be rapid, accurate, sensitive, and suitable for surveillance monitoring of food products suspected of abrin and ricin contamination.