alexa Quantitative Analysis of Tendon Structural Integrity in
ISSN: 2168-9296

Cell & Developmental Biology
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Research Article

Quantitative Analysis of Tendon Structural Integrity in Chickens using Fourier Transform-Second Harmonic Generation Imaging

Mayandi Sivaguru1, James D Richards2, Julia J Dibner2, John Paul Eichorst1,3, Terra D Wineman2 and Juxing Chen2*
1Institute for Genomic Biology, University of Illinois Urbana-Champaign, 1206 West Gregory Drive, Urbana, Illinois 61801, USA
2Novus International Inc. 20 Research Park Drive, St Charles, Missouri 63304, USA
3University of Minnesota – Twin Cities, Medicine – PACCS, 116 Church St SE, Minneapolis 55455, USA
*Corresponding Author : Juxing Chen
Novus International Inc. 20 Research Park Drive
St Charles, Missouri 63304, USA
Tel: 636-926-7418
Fax: 636-926-7449
E-mail: [email protected]
Received April 27, 2014; Accepted June 07, 2014; Published June 09, 2014
Citation: Sivaguru M, Richards JD, Dibner JJ, Eichorst JP, Wineman JD (2014) Quantitative Analysis of Tendon Structural Integrity in Chickens using Fourier Transform-Second Harmonic Generation Imaging . Cell Dev Biol 3:140. doi:10.4172/2168-9296.1000140
Copyright: © 2014 Sivaguru M, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
 

Abstract

Degenerative changes in tendons are common in human and land-dwelling vertebrates of all species, and are associated with both age and use. Tendon rupture and inflammation are common causes of lameness in broilers and breeders at multiple ages and has become a very important issue in the poultry industry attempts to analyze tendon health have relied primarily on visual assessment. The ability to conduct meaningful experiments on causes and interventions would be much improved by a direct assay of tendon structural integrity. The objective of this study is to test whether we can use Fourier transform-second harmonic generation (FT-SHG) imaging technique to quantify the differences in collagen fiber organization and nuclei morphology between normal and injured chicken tendons. Tendon injury/degeneration was induced by rearing chickens in wire-floor pens and feeding them an oxidized fat diet. Injured tendons had greater collagen fiber degradation/degeneration; more randomly organized collagen fibers, higher cellularity and more circular-shaped nuclei than normal tendons, indicating loss of collagen tertiary structure and lateral compression in injured tendons. Therefore, analysis of collagen fiber organization and nuclear morphology using FT-SHG imaging is a potential diagnostic tool to evaluate tendon structural health and to test the effects of nutrients and intervention strategies on the structural development of tendons in chickens.

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