alexa Quantitative Detection of Antibodies to Aleutian Disease Virus in Dried Blood Spots as an Estimation of Hypergammaglobulinemia in Mink
ISSN: 2161-0517

Virology & Mycology
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

Quantitative Detection of Antibodies to Aleutian Disease Virus in Dried Blood Spots as an Estimation of Hypergammaglobulinemia in Mink

Anna-Maria Andersson*, Helena Reineck-Bosaeus, Ann-Kristin Nyman and Per Wallgren

Department of Animal Health and Antimicrobial Strategies, The National Veterinary Institute, SVA, SE-751 89 Uppsala, Sweden

Corresponding Author:
Anna-Maria Andersson
Department of Animal Health and Antimicrobial Strategies
The National Veterinary Institute
SVA, SE-751 89 Uppsala, Sweden
Tel: +46 18 674000
E-mail: [email protected]

Received Date: September 24, 2015; Accepted Date: October 29, 2015; Published Date: November 12, 2015

Citation: Reineck-Bosaeus H, Nyman AK, Wallgren (2015) Quantitative Detection of Antibodies to Aleutian Disease Virus in Dried Blood Spots as an Estimation of Hypergammaglobulinemia in Mink. Virol-mycol 4:147. doi:10.4172/2161-0517.1000147

Copyright: © 2015 Andersson AM et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Infections with Aleutian disease virus (ADV) cause a progressive hypergammaglobulinemia, immune complex formation and plasma cell infiltrations in internal organs which induce a multi-systemic disease with high mortality. Serum ADV antibodies have traditionally been diagnosed with counter immunoelectrophoresis (CIEP) as a gold standard for qualitative assessment separating infected from non-infected animals, but less laborious ELISA methods have been confirmed to be equally sensitive. A way to simplify the diagnostics further could be to demonstrate antibodies in Dried blood spot samples (DBS). However, quantitative analysis of ADV antibodies in DBS and its correlation to the degree of hypergammaglobulinemia have not been scientifically published. The aim of this paper was to describe the adaptation and validation of the VP2 ELISA to ADV antibody detection in DBS and compare the estimated antibody levels in DBS to CIEP results, the estimated antibody levels and albumin: gamma globulin ratio in serum. The VP2 ELISA worked technically well when transferred from serum to DBS with mean intra-assay and mean inter-assay coefficients of variation within ± 20%. The DBS VP2 ELISA had a sensitivity of 97.3% and specificity of 93.2% compared to CIEP. Further, we found a correlation coefficient between the level of antibodies in DBS and the A:γG ratio of -0.81. The correlation between the A:γG ratio and the OD450 value was superior in DBS compared to serum samples from the same mink with the most pronounced difference at low A:γG ratios. Our results confirmed that the VP2 ELISA could detect ADV antibodies in DBS with a high sensitivity and specificity when employing CIEP as gold standard. The antibody titers estimated with DBS VP2 ELISA were well correlated to the antibody titters and A:γG ratios in serum, and the DBS VP2 ELISA could be an applicable and preferable tool for estimating AD progression in mink.

Keywords

Share This Page

Additional Info

Loading
Loading Please wait..
 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords