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Quantitative Determination of Amlodipine Besylate, Losartan Potassium, Valsartan and Atorvastatin Calcium by HPLC in their Pharmaceutical Formulations | Abstract
ISSN: 2157-7064

Journal of Chromatography & Separation Techniques
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Research Article

Quantitative Determination of Amlodipine Besylate, Losartan Potassium, Valsartan and Atorvastatin Calcium by HPLC in their Pharmaceutical Formulations

Hafez HM1*, Abdullah AE2, Abdelaziz LM2 and Kamal MM3

1Department of Pharmaceutical Science, Zagazig University, Zagazig, Egypt

2Department of Medicinal Chemistry, Faculty of Pharmacy, Zagazig University, Zagazig, Egypt

3Department of Chemistry, Nancy University, EIPICO, Egypt

*Corresponding Author:
Hafez HM
Bachelor degree of Pharmaceutical Science
Zagazig University, Zagazig, Egypt
Tel: 020113231458
E-mail: [email protected]

Received date: February 19, 2014; Accepted date: May 29, 2014; Published date: May 31, 2014

Citation: Hafez HM, Abdullah AE, Abdelaziz LM, Kamal MM (2014) Quantitative Determination of Amlodipine Besylate, Losartan Potassium, Valsartan and Atorvastatin Calcium by HPLC in their Pharmaceutical Formulations. J Chromatograph Separat Techniq 5:226 doi:10.4172/2157-7064.1000226

Copyright: © 2014 Hafez HM, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


Amlodipine besylate is a calcium channel blocker which is used in treatment of hypertension alone or in combination with other antihypertensive drugs like angiotensin-II-receptor antagonists (ARA II) group (Losartan potassium and Valsartan) or in combination with anti hyperlipidemic agent like Atorvastatin calcium. RP- HPLC method was developed for the assay of these drugs. The method was performed by reversed phase high performance liquid chromatography using a mobile phase 0.01 M ammonium acetate buffer (pH 5.5): acetonitrile with detection at 240 nm on a spherical monomeric C18 column (250 mm × 4.6 mm, 5 μm) at flow rate of 1.5 ml/min. The proposed method was validated in terms of linearity ranged between [(2-12, 10-60, 16-96, 4-24 μg/ml) corresponding levels of 20-120% w/w of the nominal analytical concentration] with linear regression equations were [{y=64.627x – 3.6383 (r= 0.9998), y=75.385x – 8.3856 (r= 0.9997), y=64.492x – 25.981 (r= 0.9998), y=70.964x – 28.505 (r= 0.9998}], accuracy [100.18 ± 1.38, 100.79 ± 0.59, 100.45 ± 0.58 and 100.8 ± 1.69%], precision [99.29, 99.33, 99.30 and 99.30%], limits of detection [0.03, 0.18, 0.15, 0.007 μg/ml] and limits of quantitation [0.1, 0.54, 0.45, 0.024 μg/ml] for Amlodipine besylate, Losartan potassium, Valsartan and Atorvastatin calcium respectively. Method validation was developed following the recommendations for analytical method validation of International Conference on Harmonization (ICH) and Food and Drug Administration (FDA) organizations.


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