Quantitative Determination of Four Angiotensin-II-Receptor Antagonistsin Presence of Hydrochlorothiazide by a Gradient Technique HPLC in their Pharmaceutical Preparations
- *Corresponding Author:
- Hani M. Hafez
Bachelor degree of Pharmaceutical Science
Zagazig University, Zagazig, Egypt
E-mail: [email protected]
Received date: July 15, 2012; Accepted date: September 03, 2012; Published date: September 07, 2012
Citation: Hafez HM, Abdelaziz LM, Elshanawane AA, Kamal MM (2012) Quantitative Determination of Four Angiotensin-II-Receptor Antagonists in Presence of Hydrochlorothiazide by a Gradient Technique HPLC in their Pharmaceutical Preparations. Pharmaceut Anal Acta 3:167. doi:10.4172/2153-2435.1000167
Copyright: © 2012 Hafez HM, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Losartan potassium, Valsartan , Telmisartan and Irbesartan are angiotensin-II-receptor antagonists (ARA II) group which used in treatment of hypertension alone or in combination with other drugs mainly Hydrochlorothiazide. A gradient HPLC method was developed for the assay of several ARAII in presence of Hydrochlorothiazide. The method was performed by reversed phase high performance liquid chromatography using a mobile phase 0.025 M potassium dihydrogen phosphate (pH 6.0): acetonitrile =80:20% with detection at 220 nm on an ACE C18 column (250 mm × 4.6 mm, 5 μm) at flow rate 1.5 ml/min in a gradient manner. The proposed method was validated in terms of linearity, accuracy, precision and limits of detection and quantitation.