alexa Quantitative Estimation of Lopinavir and Ritonavir in Tablets by RP-HPLC Method | OMICS International | Abstract
ISSN : 2153-2435

Pharmaceutica Analytica Acta
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Research Article

Quantitative Estimation of Lopinavir and Ritonavir in Tablets by RP-HPLC Method

Jagadeeswaran M1*, Gopal N2, Pavan kumar K1 and Siva kumar T1

1Nandha College of Pharmacy and Research Institute, Department of Pharmaceutical Analysis, Erode -638 052, Tamil Nadu, India

2Balaji Institute of Pharmaceutical Sciences, Department of Pharmaceutical Chemistry, Narsampet, Warangal-506 331, Andhra Pradesh, India

*Corresponding Author:
Jagadeeswaran M
Assistant Professor, Department of Pharmaceutical Analysis
Nandha College of Pharmacy and Research Institute
Perundurai Road, Erode-638 052, TamilNadu, India
E-mail: [email protected]

Received Date: February 15, 2012; Accepted Date: July 10, 2012; Published Date: July 14, 2012

Citation: Jagadeeswaran M, Gopal N, Pavan kumar K, Siva kumar T (2012) Quantitative Estimation of Lopinavir and Ritonavir in Tablets by RP-HPLC Method. Pharmaceut Anal Acta 3:160.doi: 10.4172/2153-2435.1000160

Copyright: © 2012 Jagadeeswaran M, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


A reversed phase high-performance liquid chromatographic method was developed and validated for the quantitative determination of two antiviral drugs viz. lopinavir and ritonavir. Chromatography was carried out by gradient technique on a reversed-phase C18 Column, Phenomenex (250 x 4.6 mm, 5 μ) with mobile phase mixture of Buffer: Acetonitrile (45:55 v/v) was used as a mobile phase and the pH was adjusted into 4.5 by using with O-phosphoric acid, at a flow rate of 1.2 ml/min. The UV range was detected at 240 nm for lopinavir and ritonavir respectively. The different analytical performance parameters such as linearity, precision, accuracy, specificity, limit of detection (LOD) and limit of quantification (LOQ) were determined according to International Conference on Harmonization ICH Q2B guidelines. The linearity of the calibration curves for each analyte in the desired concentration range is good (r2 >0.9). The recovery of the method was between 102.1% and 100.1% for lopinavir and ritonavir respectively. Hence the proposed method is highly sensitive, precise and accurate and it successfully applied for the reliable quantification of API content in the commercial formulations of lopinavir and ritonavir.


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