alexa Quantitative Profiling of Histone H3 Methylation in Hum
ISSN: 0974-276X

Journal of Proteomics & Bioinformatics
Open Access

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Research Article

Quantitative Profiling of Histone H3 Methylation in Human Hepatocellular Carcinoma

Jiajia Chen1,2, Guoquan Yan1, Fang Wang1,2, Xuefei Yin1,2, Hong Jin1,2* and Peng-yuan Yang1,2*

1Department of Chemistry, Fudan University, 220 Handan Road, Shanghai 200433, China

2Institute of Biomedical Science, Fudan University, 138 Yixueyuan Road, Shanghai, 200032, China

*Corresponding Author:
Hong Jin
Department of Chemistry
Fudan University
220 Handan Road
Shanghai 200433, China
Tel: 0086 2154237664
Fax:0086 21 54237961
E-mail: [email protected]

Peng-yuan Yang
Institute of Biomedical Science
Fudan University, 138 Yixueyuan
Road, Shanghai, 200032, China
Tel: 0086 2165642009
Fax:0086 21 65642009
E-mail: [email protected]

Received Date: May 10, 2013; Accepted Date: June 10, 2013; Published Date: June 13, 2013

Citation: Chen J, Yan G, Wang F, Yin X, Jin H, et al. (2013) Quantitative Profiling of Histone H3 Methylation in Human Hepatocellular Carcinoma. J Proteomics Bioinform S2: 003. doi:10.4172/jpb.S2-003

Copyright: © 2013 Chen J, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.



Alterations of epigenetics including DNA methylation and histone modifications play crucial roles in both initiation and progression of certain types of cancers. Abnormal histone modifications identified within these tumors represent potential biomarkers and therapeutic pathways. In this study, two different types of human hepatocellular carcinoma cell lines HepG2 and MHCC97-H with distinct metastasis capability are used to investigate the association of liver metastasis with cellular histone modification levels. In the HepG2 and MHCC97-H cells, 17 types of histone H3 modification corresponding to methylation are quantitatively profiled with LC coupled to high resolution mass spectrometry. Compared to HepG2 cells without metastasis capability, the combinatorial histone peptides of K9me1K14ac and K9me0K14ac in malignant MHCC97-H cells are found to be significantly down regulated, while the level of H3K9me3 and H3K27me2 are greatly increased. Overall these data provide novel insights into the histone methylation regulatory mechanism in liver metastasis and reveal the epigenetic pathway for HCC disease development.


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