alexa Range Charts for Agreement in Measurement Comparison Studies, With Application to Replicate Mass Spectrometry Experiments | OMICS International | Abstract
ISSN: 0974-276X

Journal of Proteomics & Bioinformatics
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Research Article

Range Charts for Agreement in Measurement Comparison Studies, With Application to Replicate Mass Spectrometry Experiments

James A. Koziol1*, Anne C. Feng1, Jingyi Yu2, Noelle M. Griffin2,Jan E. chnitzer2

1Department of Molecular and Experimental Medicine, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037

2Sidney Kimmel Cancer Center, 10905 Road to the Cure, San Diego, CA 92122

*Corresponding Author:
Dr. James A. Koziol
Ph.D, Department of Molecular and Experimental Medicine
MEM216, The Scripps Research Institute, 10550 North Torrey
Pines Road, La Jolla, CA 92037,
Tel : 858-784-2703
Fax: 858-784-2664
E-mail: [email protected]

Received Date: August 02, 2008; Accepted Date: September 02, 2008; Published Date: September 13, 2008

Citation: James AK, Anne CF, Jingyi Y, Noelle MG, Jan ES (2008) Range Charts for Agreement in Measurement Comparison Studies, With Application to Replicate Mass Spectrometry Experiments. J Proteomics Bioinform 1:287-292. doi:10.4172/jpb.1000036

Copyright: © 2008 James AK, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

It is important to investigate the reproducibility of raw mass spectrometry (MS) features of abundance, such as spectral count, peptide number and ion intensity values, when conducting replicate mass spectrometry measurements. Reproducibility can be inferred from these replicate data either formally with analyses of variance techniques or informally with graphical procedures, particularly, Bland-Altman plots on paired runs. In this note, we suggest range plots to provide a suitable generalization of Bland-Altman plots to experiments with more than two replicate runs. We describe range charts and their interpretation, and illustrate their use with data from a recent proteomic study relating to label-free analysis.

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