alexa Rapid and Specific Approach for Direct Measurement of T
ISSN: 1948-593X

Journal of Bioanalysis & Biomedicine
Open Access

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Research Article

Rapid and Specific Approach for Direct Measurement of Topiramate in Human Plasma by LC-MS/MS: Application for Bioequivalence Study

S. R. Kuchekar 1*, M. L. Kundlik1 and B. H. Zaware2

1Padmashri Vikhe Patil College, Pravaranagar, Loni Kurd, Pin-413713, Dis- Ahmednagar, Maharastra State, India

2New Arts, Commerce and Science college of Ahmedanagar-414 001, Maharashtra State, India

Corresponding Author:
S. R. Kuchekar
Padmashri Vikhe Patil College
Pravaranagar, Loni Kurd
Pin-413713, Dis- Ahmednagar
Maharastra State, India
Tel: +91-02422-273425
Fax: +91- 02422-273426
E-mail: [email protected]

Received date: August 08, 2010; Accepted date: September 09, 2010; Published date: September 09, 2010

Citation: Kuchekar SR, Kundlik ML, Zaware BH (2010) Rapid and Specifi c Approach for Direct Measurement of Topiramate in Human Plasma by LCMS/ MS: Application for Bioequivalence Study. J Bioanal Biomed 2: 107-112. doi: 10.4172/1948-593X.1000032

Copyright: © 2010 Kuchekar SR, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

Abstract

A rapid liquid chromatography coupled with electrospray ionization tandem mass spectrometry (LC-MS/MS) method was developed and validated for the quanti fi cation of topiramate in heparinized human plasma. The plasma samples were prepared by solid phase extraction (SPE) method without drying and then reconstitution. Topiramate and the topiramate d12 (Internal Standard IS) were chromatographed on a Betasil C18 column at a fl ow rate of 0.5 ml/min. The total run time was 1.80 min. An electrospray ionization interface was selected for ionization of analyte and IS. The mass transition [M-H] ions used for detection were m/z 338.10 → 78.20 for topiramate, m/z 350.40 → 90.10 for IS. The method was linear in the concentration range of 10–4200 ng/ml with r ≥ 0.9982. Recovery of topiramate and IS ranged from 78.20 to 87.74%. The validated method has been successfully used to analyze human plasma samples for application in 100 mg fasted pharmacokinetic studies.

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