Rapid Detection of Sporotrichosis by ABC-ELISA
- *Corresponding Author:
- Xianling Cong
Department of Dermatology and Venereology
Hospital of Jilin University
Changchun 130033, China
E-mail: [email protected]
Received date: August 11, 2012; Accepted date: October 23, 2012; Published date: November 03, 2012
Citation: Zhang Y, Zu J, Pyla V, Yue Q, Cong X (2012) Rapid Detection of Sporotrichosis by ABC-ELISA. J Clin Exp Dermatol Res 3:162. doi: 10.4172/2155-9554.1000162
Copyright: ©2012 Zhang Y, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Background: Avidin-biotin-horseradish peroxidase enzyme complex, enzyme-linked immunosorbent assay (ABCELISA) is widely used in detecting the soluble bacterial, viral antigen and antibody, but the reports of detecting mycotic antigens were few .We used the method to detect Sporothrix schenckii pathogen of sporotrichosis in smear.
Aim: To provide a rapid diagnostic method for sporotrichosis.
Methods: 20 cases of sporotrichosis-in doubt we scraped pus or tissue fluid from the skin lesion and perform ABCELISA by reagent vectastatin ABC kit, rabbit anti Sporothrix schenckii serum.
Results: 18 of 20 cases were diagnosed as sporotrichosis. 14 of 18 cases were positive (77.8%) by ABC-ELISA stain, 5 of 18 cases were positive (27.7%) by Gram stain.
Conclusion: ABC ELISA is new fashioned biological amplification technique due to its sensitivity of this method is 77.8% in smear, the specificity is 100%. Only three hours is needed, so this method can be used to detect Sporothrix shenckii rapidly.