alexa Rapid Quantitative Evaluation of Amphotericin B in Human Plasma, by Validated HPLC Method
ISSN: 0975-0851

Journal of Bioequivalence & Bioavailability
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

Rapid Quantitative Evaluation of Amphotericin B in Human Plasma, by Validated HPLC Method

Pavan Balabathula1*, Dileep R Janagam2, Nivesh K Mittal1, Bivash Mandal1, Laura A Thoma1 and George C Wood1

1Plough Center for Sterile Drug Delivery Systems, Department of Pharmaceutical Sciences, College of Pharmacy, University of Tennessee Health Science Center, Memphis, TN, USA

2Department of Pharmaceutical Sciences, College of Pharmacy, University of Tennessee Health Science Center, Memphis, TN, USA

*Corresponding Author:
Pavan Balabathula
26 S Dunlap St. Suite 214 Memphis
TN – 38163, USA
Tel: +001-901-448-4637
Fax: +001-901-448-6092
E-mail: [email protected]

Received Date: April 04, 2013; Accepted Date: April 27, 2013; Published Date: May 03, 2013

Citation: Balabathula P, Janagam DR, Mittal NK, Mandal B, Thoma LA, et al. (2013) Rapid Quantitative Evaluation of Amphotericin B in Human Plasma, by Validated HPLC Method. J Bioequiv Availab 5:121-124. doi: 10.4172/jbb.1000145

Copyright: © 2013 Balabathula P, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

Abstract

A simple, rapid, and sensitive HPLC method was developed and validated to quantify Amphotericin B (AmB) in human plasma. AmB was extracted from spiked plasma by simple protein precipitation with methanol. The separation was performed on an XBridge TM C18 (150 × 4.6 mm, 3.5 μm) column, with a mobile phase of acetic acid (0.73%) – acetonitrile (60:40, v/v) and at a flow rate of 1 mL/min. The eluted peak of AmB was monitored at 408 nm with photo-diode array detector (PDA) detector. The calibration curve was found linear in the AmB concentration range of 1000 – 50 ng/mL (r 2 >0.99). The inter- and intra-day precisions (%CV) were less than 11.2%. The extraction recoveries were 85-91%. The method developed and validated is simple, rapid (<3 min per injection), sensitive, and reproducible. It potentially can be used for the pharmacokinetic, bioequivalence, and toxicokinetic studies of AmB.

Keywords

Share This Page

Additional Info

Loading
Loading Please wait..
 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

[email protected]

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

[email protected]

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001 Extn: 9042

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords