Rapid Quantitative Evaluation of Amphotericin B in Human Plasma, by Validated HPLC Method
- *Corresponding Author:
- Pavan Balabathula
26 S Dunlap St. Suite 214 Memphis
TN – 38163, USA
E-mail: [email protected]
Received Date: April 04, 2013; Accepted Date: April 27, 2013; Published Date: May 03, 2013
Citation: Balabathula P, Janagam DR, Mittal NK, Mandal B, Thoma LA, et al. (2013) Rapid Quantitative Evaluation of Amphotericin B in Human Plasma, by Validated HPLC Method. J Bioequiv Availab 5:121-124. doi: 10.4172/jbb.1000145
Copyright: © 2013 Balabathula P, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
A simple, rapid, and sensitive HPLC method was developed and validated to quantify Amphotericin B (AmB) in human plasma. AmB was extracted from spiked plasma by simple protein precipitation with methanol. The separation was performed on an XBridge TM C18 (150 × 4.6 mm, 3.5 μm) column, with a mobile phase of acetic acid (0.73%) – acetonitrile (60:40, v/v) and at a flow rate of 1 mL/min. The eluted peak of AmB was monitored at 408 nm with photo-diode array detector (PDA) detector. The calibration curve was found linear in the AmB concentration range of 1000 – 50 ng/mL (r 2 >0.99). The inter- and intra-day precisions (%CV) were less than 11.2%. The extraction recoveries were 85-91%. The method developed and validated is simple, rapid (<3 min per injection), sensitive, and reproducible. It potentially can be used for the pharmacokinetic, bioequivalence, and toxicokinetic studies of AmB.