alexa Rapid Quantitative Evaluation of Amphotericin B in Human Plasma, by Validated HPLC Method | OMICS International | Abstract
ISSN: 0975-0851

Journal of Bioequivalence & Bioavailability
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Research Article

Rapid Quantitative Evaluation of Amphotericin B in Human Plasma, by Validated HPLC Method

Pavan Balabathula1*, Dileep R Janagam2, Nivesh K Mittal1, Bivash Mandal1, Laura A Thoma1 and George C Wood1

1Plough Center for Sterile Drug Delivery Systems, Department of Pharmaceutical Sciences, College of Pharmacy, University of Tennessee Health Science Center, Memphis, TN, USA

2Department of Pharmaceutical Sciences, College of Pharmacy, University of Tennessee Health Science Center, Memphis, TN, USA

*Corresponding Author:
Pavan Balabathula
26 S Dunlap St. Suite 214 Memphis
TN – 38163, USA
Tel: +001-901-448-4637
Fax: +001-901-448-6092
E-mail: [email protected]

Received Date: April 04, 2013; Accepted Date: April 27, 2013; Published Date: May 03, 2013

Citation: Balabathula P, Janagam DR, Mittal NK, Mandal B, Thoma LA, et al. (2013) Rapid Quantitative Evaluation of Amphotericin B in Human Plasma, by Validated HPLC Method. J Bioequiv Availab 5:121-124. doi: 10.4172/jbb.1000145

Copyright: © 2013 Balabathula P, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

A simple, rapid, and sensitive HPLC method was developed and validated to quantify Amphotericin B (AmB) in human plasma. AmB was extracted from spiked plasma by simple protein precipitation with methanol. The separation was performed on an XBridge TM C18 (150 × 4.6 mm, 3.5 μm) column, with a mobile phase of acetic acid (0.73%) – acetonitrile (60:40, v/v) and at a flow rate of 1 mL/min. The eluted peak of AmB was monitored at 408 nm with photo-diode array detector (PDA) detector. The calibration curve was found linear in the AmB concentration range of 1000 – 50 ng/mL (r 2 >0.99). The inter- and intra-day precisions (%CV) were less than 11.2%. The extraction recoveries were 85-91%. The method developed and validated is simple, rapid (<3 min per injection), sensitive, and reproducible. It potentially can be used for the pharmacokinetic, bioequivalence, and toxicokinetic studies of AmB.

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