Rate of Formation of Glycated Albumin Revisited and Clinical ImplicationsMargo P. Cohen*, Elizabeth Hud and Elizabeth Shea
Glycadia, Inc, Philadelphia, PA, USA
- *Corresponding Author:
- Margo P. Cohen, M.D
1880 JFK Boulevard, Suite 200, Philadelphia, PA 19103
E-mail: drmpcohe[email protected]
Received date: July 28, 2010; Accepted date: August 27, 2010; Published date: August 27, 2010
Citation: Cohen MP, Hud E, Shea E (2010) Rate of Formation of Glycated Albumin Revisited and Clinical Implications. J Diabetes Metab 1:102. doi: 10.4172/2155-6156.1000102
Copyright: © 2010 Cohen MP, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Background: Albumin modified by Amadori glucose adducts contributes to the pathogenesis of complications of diabetes and reducing its formation ameliorates their development, underscoring the need for accurate information on the rate of formation of this biologically active glycated protein. However, this subject has not been examined in over two decades, and there is reason to question data in older reports. Methods: The present study used nonradioactive and non reductive techniques to examine the rate of formation of glycated albumin and compare it to that of glycohemoglobin, using a sensitive and specifi c immunoassay for measurement of the stable glucose adduct formed after incubation of serum, plasma and purified albumin with glucose. Results and Conclusions: We report that the rate of formation of glycated albumin parallels that of glycohemoglobin at approximately 0.005-0.008 percent per mM glucose per day, refuting values from the older literature and providing clinically relevant information concerning levels of glycated albumin in diabetes and of treatment directed at reducing its formation.