Real-time PCR in Clinical Diagnostic Settings
Department of Medical Microbiology and Immunology, University of California Davis, Davis, California, USA
- *Corresponding Author:
- Sumathi Sankaran-Walters
Department of Medical Microbiology and Immunology
University of California Davis
Davis, California, USA
E-mail: [email protected]
Received Date: May 08, 2012; Accepted Date: May 09, 2012; Published Date: May 14, 2012
Citation: Walters SS (2012) Real-time PCR in Clinical Diagnostic Settings. J Med Microb Diagn 1:e106. doi: 10.4172/2161-0703.1000e106
Copyright: © 2012 Walters SS. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Real-time PCR is a commonly used technique for the detection of pathogens both in research setting, as well as in diagnostic settings. The goal of this editorial is to introduce or familiarize the reader with the advantages of using the available methodologies in a targeted manner. Real-time PCR combines PCR chemistry with either a fluorescent probe or DNA detection dyes such as syber green, thus allowing visualization of product in "real-time". The available real-time PCR methodologies provide good sensitivity and specificity. The ease and speed of the design and setup process of qPCR makes it an attractive assay to use. PCR amplification is performed in a closed setting (one tube vs two tube) thus reducing the chances of contamination.