Reach Us +44-1647-403003
Real-time PCR in Clinical Diagnostic Settings | OMICS International | Abstract
ISSN: 2161-0703

Journal of Medical Microbiology & Diagnosis
Open Access

Like us on:

Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)


Real-time PCR in Clinical Diagnostic Settings

Sumathi Sankaran-Walters*

Department of Medical Microbiology and Immunology, University of California Davis, Davis, California, USA

*Corresponding Author:
Sumathi Sankaran-Walters
Department of Medical Microbiology and Immunology
University of California Davis
Davis, California, USA
E-mail: [email protected]

Received Date: May 08, 2012; Accepted Date: May 09, 2012; Published Date: May 14, 2012

Citation: Walters SS (2012) Real-time PCR in Clinical Diagnostic Settings. J Med Microb Diagn 1:e106. doi: 10.4172/2161-0703.1000e106

Copyright: © 2012 Walters SS. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


Real-time PCR is a commonly used technique for the detection of pathogens both in research setting, as well as in diagnostic settings. The goal of this editorial is to introduce or familiarize the reader with the advantages of using the available methodologies in a targeted manner. Real-time PCR combines PCR chemistry with either a fluorescent probe or DNA detection dyes such as syber green, thus allowing visualization of product in "real-time". The available real-time PCR methodologies provide good sensitivity and specificity. The ease and speed of the design and setup process of qPCR makes it an attractive assay to use. PCR amplification is performed in a closed setting (one tube vs two tube) thus reducing the chances of contamination.

Recommended Conferences
Share This Page