Redox-active Microcapsules as Drug Delivery System in Breast Cancer Cells and Spheroids
- Corresponding Author:
- Annarita Stringaro
Department of Technology and Health
Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Rome, Italy
E-mail: [email protected]
Received date: October 15, 2015; Accepted date: January 20, 2016; Published date: January 27, 2016
Citation: Colone M, Kaliappan S, Calcabrini A, Tortora M, Cavalieri F, et al. (2016) Redox-active Microcapsules as Drug Delivery System in Breast Cancer Cells and Spheroids. J Mol Genet Med 10:200. doi:10.4172/1747-0862.1000200
Copyright: © 2016 Colone M, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
The purpose of our study was to develop new delivery systems for drugs effective against breast cancer by using biodegradable and biocompatible capsules. Redox-active microcapsules based on thiolated polymethacrylic acid (PMA) were employed. The interaction of these PMASH capsules with breast cancer cells and the mechanism of their internalization was investigated. PMASH biocompatibility was evaluated by MTT assay. To analyze their potential as drug carrier, we incorporated doxorubicin into the capsules. Confocal microscopy observations showed the presence of capsules inside the cells. Although some drug molecules still appeared co-localized with PMASH capsules, strong doxorubicin fluorescence was observed both in the cytoplasm and nucleus, indicating the disassembling of the capsule into PMASH-drug conjugate after internalization. These results were confirmed by both flow cytometry (time course of capsule uptake) and scanning electron microscopy. PMASH capsules were also internalized in 3D cell structures (spheroids) suggesting their potential use as drug delivery system for treatment of human diseases.