Regenerated Cellulose Fiber and Film Immobilized with Lysozyme
- *Corresponding Author:
- Jonathan Y Chen
School of Human Ecology, The University
of Texas at Austin, Austin, TX 78712, USA
E-mail: [email protected]
Received date: February 27, 2014; Accepted date: June 27, 2014; Published date: July 04, 2014
Citation: Chen JY, Sun L, Edwards VJ (2014) Regenerated Cellulose Fiber and Film Immobilized with Lysozyme. Bioceram Dev Appl 4:078. doi:10.4172/2090-5025.1000078
Copyright: © 2014 Chen JY, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
The present work reports an initial engineering approach for fabricating lysozyme-bound regenerated cellulose fiber and film. Glycine-esterified cotton was dissolved in an ionic liquid solvent 1–Butyl–3–methylimidazolium Chloride (BMIMCl) in which lysozyme was activated and covalently attached to cotton cellulose through an enzymatic conjugation between its carboxyl groups and glycine cellulose’s amino groups. The resulting solution was extruded for fiber/film formation in a water bath. After performing a bicinchoninic acid (BCA) protein assay, quantity of attached lysozyme to cellulose fiber/film was evaluated. The study exhibited that a synthesis of lysozyme conjugation on cellulose in BMIMCl could be completed in a control manor, resulting in a cellulose solution suitable for fiber/film production. It was also found that lysozyme could be successfully immobilized onto the cellulose fiber and film regenerated from solution spinning with a reasonable amount ranging from 197.6 to 343.7 μg/mL.mg.