alexa Regulation of Pigment Epithelium-Derived Factor (PEDF), an Insulin Resistance-Inducing Adipocytokine, in 3T3-L1 Adipocytes | OMICS International | Abstract
ISSN: 2155-6156

Journal of Diabetes & Metabolism
Open Access

Like us on:

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

Regulation of Pigment Epithelium-Derived Factor (PEDF), an Insulin Resistance-Inducing Adipocytokine, in 3T3-L1 Adipocytes

Daisuke Ito, Kouichi Inukai*, Takashi Sumita, Hiraku Ono, Shigehiro Katayama and Takuya Awata

Division of Endocrinology and Diabetes, Department of Medicine, Saitama Medical School, Morohongo 38, Moroyama, Iruma-gun, Saitama, 350-0495, Japan

*Corresponding Author:
Kouichi Inukai
Division of Endocrinology and Diabetes
Department of Medicine, Saitama Medical School
Morohongo 38, Moroyama, Iruma-gun, Saitama, 350-0495, Japan
Fax: +81-492-76-1430
E-mail: [email protected]

Received date October 03, 2011; Accepted date November 19, 2011; Published date November 23, 2011

Citation: Ito D, Inukai K, Sumita T, Ono H, Katayama S, et al. (2011) Regulation of Pigment Epithelium-Derived Factor (PEDF), an Insulin Resistance-Inducing Adipocytokine, in 3T3-L1 Adipocytes. J Diabetes Metab 2:151. doi:10.4172/2155-6156.1000151

Copyright: © 2011 Ito D, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Pigment epithelium-derived factor (PEDF), secreted by adipocytes, was recently demonstrated to reduce insulin sensitivity and to possibly cause insulin resistance in obesity. In the present study, we investigated the regulation of adipocyte-expressing PEDF to clarify the underlying link between obesity and PEDF expression. We examined PEDF expression in 3T3-L1 adipocytes at both the transcriptional and the protein level using RT-PCR and western blot. First, we examined PEDF expression during 3T3-L1 adipocyte differentiation. PEDF expression peaked in 3T3-L1 fibroblasts and was lowest at 2 days after initiating the induction of differentiation into adipocytes (day 2). Then, PEDF expressions gradually rose until day 10, in parallel with adipocyte differentiation. Exposure of 3T3-L1 adipocytes to a PPARα or PPARγ activator reduced PEDF expression, suggesting these suppressions of PEDF expression to be involved in the mechanism by which PPAR agonists reduce insulin resistance. When 3T3-L1 adipocytes were treated with 0.5 μM H 2 O 2 , PEDF levels were markedly reduced, indicating PEDF expression to be inhibited by oxidative stress. We demonstrated PEDF expression to increase in parallel with adipocyte differentiation and to be negatively regulated by oxidative stress and PPAR agonists.

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2018-19
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

engineer[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2018 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
Leave Your Message 24x7