alexa Reliable and Sensitive SPE-HPLC-DAD Screening of Endocrine Disruptors Atrazine, Simazine and their Major Multiresidues in Natural Surface Waters: Analytical Validation and Robustness Study Perfomance
ISSN: 2157-7064

Journal of Chromatography & Separation Techniques
Open Access

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Research Article

Reliable and Sensitive SPE-HPLC-DAD Screening of Endocrine Disruptors Atrazine, Simazine and their Major Multiresidues in Natural Surface Waters: Analytical Validation and Robustness Study Perfomance

Juan José Berzas Nevado, Carmen Guiberteau-Cabanillas, María Jesus Villasenor Llerena* and Virginia Rodríguez-Robledo

Department of Analytical Chemistry and Food Technology, University of Castilla-La Mancha, 13071 Ciudad Real, Spain

*Corresponding Author:
María Jesus Villasenor Llerena
Departamento de Química Analíticay Tecnología de Alimentos
Universidad de Castilla-La Mancha, Spain
Tel: 34 926295318
E-mail: [email protected]

Received date: February 20, 2014; Accepted date: March 28, 2014; Published date: March 31, 2014

Citation: Nevado JJB, Guiberteau-Cabanillas C, Llerena MJV, Rodríguez- Robledo V (2014) Reliable and Sensitive SPE-HPLC-DAD Screening of Endocrine Disruptors Atrazine, Simazine and their Major Multiresidues in Natural Surface Waters: Analytical Validation and Robustness Study Perfomance. J Chromatograph Separat Techniq 5:215. doi:10.4172/2157-7064.1000215

Copyright: © 2014 Nevado JJB, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

Abstract

An analytical method combining off-line solid phase extraction (SPE) and reversed phase high performance liquid chromatography (HPLC) has been developed for simultaneous determination of six herbicides. The compounds analyzed in natural surface water samples, were: the herbicides atrazine (AT), simazine (SI) and their major dealkylated and hydroxylated degradation products such as deisopropyl-atrazine (DIA), deethyl-atrazine (DEA), hydroxyl-atrazine (H-AT) and hydroxyl-simazine (H-SI), all of them with herbicide activity too. As optimum conditions, a Kromasil C18 column as stationary phase, a mixture of acetonitrile and phosphate buffer pH 7.0 as mobile phase in gradient elution mode and 215 nm as measuring wavelength, were selected. A polymeric cartridge (Bond-Elut ENV-1000 mg) was used for a SPE procedure. The optimized off-line SPE HPLC-DAD method was evaluated in terms of validation and robustness procedure, obtaining among other parameters, limits of quantification between 81 and 100 ng L-1, considering the enrichment factor for all of the studied herbicides. The proposed method was successfully applied to the screening of herbicides in natural surface water samples from different locations in the 23th aquifer (Albacete, Spain).

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