alexa Repair of Excitotoxic Neuronal Damage Mediated by Neura
ISSN: 2157-7013

Journal of Cell Science & Therapy
Open Access

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Research Article

Repair of Excitotoxic Neuronal Damage Mediated by Neural Stem Cell Lysates in Adult Mice

Lijian Yu1*, Juan Ma1, Rundi Ma1, Yongping Zhang1, Xiaoyu Zhang1,3 and Tingxi Yu1,2*
1Key Laboratory of Marine Materia Medica, Guangdong Ocean University, Jiefang East Avenue 40, Xiashan, Zhanjiang 524025, China
2Cell Biology Group, Department of Surgery, Department of Pathology, University of Maryland School of Medicine and Baltimore Veterans Affairs Medical Center, Baltimore, MD 21201, USA
3Department of Otorhinolaryngology-Head and Neck Surgery, University of Maryland School of Medicine, MD 21228), USA
Corresponding Authors : Prof. Lijian Yu
Key Laboratory of Marine Materia Medica
Guangdong, Ocean University, Jiefang East Avenue 40
Xiashan, Zhanjiang 524025, China
Tel: 86-759-2382041
Fax: 86-759-2382424
E-mail:[email protected]
  Dr. Tingxi Yu, PhD
Cell Biology Group, Department of Surgery,Department of Pathology
University of Maryland School of Medicine and Baltimore Veterans Affairs, USA
Tel: 001-410-660-9557
Fax: 001-410-747-5062
E-mail:[email protected]
Received August 16, 2011; Accepted October 11, 2011; Published October 13, 2011
Citation: Yu L, Ma J, Ma R, Zhang Y, Zhang X, et al. (2011) Repair of Excitotoxic Neuronal Damage Mediated by Neural Stem Cell Lysates in Adult Mice. J Cell Sci Ther 2:109. doi: 10.4172/2157-7013.1000109
Copyright: © 2011 Yu L, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


The present study aimed to investigate a possibility of brain repair from diseased or damaged disorders mediated by cell-free filtrate of neural stem cell (NSC) lysates (FNSCL). Mouse NSCs were isolated from the brains of embryos at 15 day postcoitum (dpc). The expression of the Nestin was examined by immunocytochemical technique. Sonication of NSCs cultured and nestin-positive was performed in a bath-type sonicator, and the cell-free filtrate was recovered from a filtrative step of the lysates. The animals in the monosodium glutamate MSG group received intragastric(ig) administration of MSG (2.0 g/kg per day) for 10 days the animals in the MSG+NSCs and MSG+FNSCL groups received intracerebroventricular transplantation of 10 ?l of NSC suspension, approximately 1.0×105 cells, or intracerebroventricular injection of 10 ?l of cell-free filtrate of lysates of approximately 1.0×105 NSCs on day 1 and day 11 after 10-d MSG exposure, respectively. The mice in control and MSG group were intracerebroventricularly administered with 10 ?l of DMEM instead of NSCs or FNSCL. On 11 day after intracerebroventricular transplantation of NSCs or intracerebroventricular injection of FNSCL, the test of Y-maze discrimination learning were performed, and then the histopathology of the animal brains was studied, to analyze MSG-induced functional and morphological changes and the effects of intracerebroventricular transplantation of NSCs or intracerebroventricular injection of FNSCL on the brain repair from MSG-induced excitotoxic injury. Both intracerebroventricular transplantation of NSCs and intracerebroventricular injection of FNSCL facilitated the brain repair following glutamate-induced excitotoxic injury in adult mice, suggesting that there are certain NSC factors inside NSCs which are effective in repairing glutamate-induced excitotoxic brain injury. Administration of FNSCL might be a cell-free-based therapeutic strategy to repair diseased or damaged central nervous system CNS tissue.


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