Report of Interaction Between Calf Thymus DNA and Pyrimidine-Annulated Spiro-DihydrofuranSwarup Roy1*, Sintu Ganai2, Raj Kumar Nandi2, K.C. Majundar2 and Tapan K. Das1
- Corresponding Author:
- Swarup Roy
Department of Biochemistry and Biophysics
University of Kalyani, West Bengal, India
Tel: (033) 25828750, 2582 8378 (Ext. 385) +91-9046546632
E-mail: [email protected] com, [email protected]
Received Date: February 03, 2016; Accepted Date: May 31, 2016; Published Date: June 03, 2016
Citation: Roy S, Ganai S, Nandi RK, Majundar KC, Das TK (2016) Report of Interaction Between Calf Thymus DNA and Pyrimidine-Annulated Spiro- Dihydrofuran. Biochem Anal Biochem 5:278. doi:10.4172/2161-1009.1000278
Copyright: © 2016 Roy S, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
The study of binding interaction between pyrimidine-annulated spiro-dihydrofuran (PSDF) with calf thymus DNA (CTDNA) using UV absorption, fluorescence, circular dichroism (CD), and molecular docking methods. The experimental results revealed that PSDF preferred to bind to the groove of CTDNA with the binding constant (K) of 1.51×102 L/mol at 293 K. Based on the signs and magnitudes of the enthalpy change (ΔH = -33.25 kJ/mol) and entropy change (ΔS= -71.58 J/mol/K) in the binding process it can be concluded that the main interaction forces between PSDF and CTDNA in the binding process were van der Waals force and hydrogen bonding interaction. The results of CD experiments revealed that PSDF did not disturb native conformation of CTDNA and the significant binding of PSDF in PSDF-CTDNA complex was observed from the molecular docking results.