Reproductive Toxicity of Lead Acetate in Adult male Rats: Histopathological and Cytotoxic StudiesZohra Haouas1*, Ines Zidi1, Amira Sallem1, Rakia Bhouri1, Tesnim Ajina1, Monia Zaouali2 and Meriem Mehdi1
- *Corresponding Author:
- Zohra Haouas
Laboratory of Histology
Embryology and Cytogenetic (UR 12 ES 10)
Faculty of Medicine, 5019 Monastir
University of Monastir, Tunisia
Tel: 216 73 462200
E-mail: [email protected]
Received date: November 03, 2014; Accepted date: December 15, 2014; Published date:December 17, 2014
Citation: Haouas Z, Zidi I, Sallem A, Bhouri R, Ajina T, et al. (2015) Reproductive Toxicity of Lead Acetate in Adult male Rats: Histopathological and Cytotoxic Studies. J Cytol Histol 6:293. doi:10.4172/2157-7099.1000293
Copyright: © 2015 Haouas Z, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
The present study was conducted to assess the histopathological and cytotoxic effect of lead exposure on male reproductive function. Adult male rats were divided in 2 groups. The first as a control group was given distilled water as drinking, while the second was exposed to 2 g/l of lead acetate in distilled water during 35 days. The structural damage on tests parenchyma was investigated by histological study and supplemented by biochemical assay of testosterone level. DNA fragmentation in germinal cells was determined using terminal desoxynucleotidyl transferase mediated dUTP nick end-labeling (TUNEL) assay. The histological study showed that lead can induce pronounced alterations on germ cells. The testosterone level was significantly decreased in treated rats compared to controls. TUNEL assay revealed a significant DNA fragmentation index in rats exposed to lead. This study showed TUNEL assay can be used to determinate DNA fragmentation in germinal cells. Moreover, we conclude that lead acetate may be considered as strong cytotoxic and genotoxic agent on male reproductive function.