RESPONSE OF ANTIOXIDATIVE ENZYMES AND LIPOXYGENASE TO DROUGHT STRESS IN FINGER MILLET LEAVES (ELEUSINE CORACANA (L.) GAERTN)
Kasi Viswanath Kotapati1., Bhagath Kumar Palaka1., Anithamma Kandukuri2., Ramachandra Reddy
Pamuru2., Dinakara Rao Ampasala1*
|Corresponding Author: Dinakara Rao Ampasala, E-mail: [email protected]|
|Received: 20 June-2014 Accepted: 17 July-2014|
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Millets are minor cereals of the grass family, Poaceae. Ragi or finger millet (Eleusine coracana L) is one of the common millets in several regions of India. The productivity of crop is greatly affected by various environmental stress. The current study presents the impact of drought stress on various antioxidant enzymes and on Lipoxygenase activity during finger millet plant growth (drought-tolerant, Sri Chaitanya VR-847). The possible involvement of activated oxygen species in drought stress was studied in finger millet leaves. The changes in H2O2, free proline content, and the rate of lipid peroxidation level in terms of malondialdehyde (MDA) in millet leaves was investigated in response to drought tolerance. Increased levels of MDA and higher amount of proline levels were observed under drought stress in the leaves. Antioxidant enzymes such as SOD, CAT, APX and GR showed elevated activities with drought stress in millet leaves. Lipoxygenases involved in many physiological and pathological activities and produces the reactive oxygen species by oxidizing unsaturated fatty acids which may be lethal to plant if present in excess. A significant increase in Lipoxygenase enzyme activity and its gene expression observed with water deficit condition in millets. The results indicate that improved tolerance to drought stress in millet crop may accomplished by increased capacity of lipoxygenase and antioxidative system for better drought tolerance in this cultivar.