alexa RNAi Induced Wing Modification in Leon Mutant Drosophila Females: Exploring the Tissue Specificity and Physiological Interference
ISSN: 2329-6682

Gene Technology
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Research Article

RNAi Induced Wing Modification in Leon Mutant Drosophila Females: Exploring the Tissue Specificity and Physiological Interference

Sandeep Satapathy1* and Cheng Ting Chien2
1Department of Biological Sciences, Indian Institute of Science Education and Research, Bhopal, India
2Department of Neurosciences, Institute of Molecular Biology (IMB), Academia Sinica, Taiwan, R.O.C, Taiwan
Corresponding Author : Sandeep Satapathy
Department of Biological Sciences
Indian Institute of Science Education and Research
Bhopal, India
Tel: 91-7898664419
E-mail: [email protected]
Received September 03, 2013; Accepted September 13, 2013; Published September 20, 2013
Citation: Satapathy S, Chien CT (2013) RNAi Induced Wing Modification in Leon Mutant Drosophila Females: Exploring the Tissue Specificity and Physiological Interference. Gene Technology 2:104. doi:10.4172/2329-6682.1000104
Copyright: © 2013 Satapathy S, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
 

Abstract

The precision of growth of an animal is meticulously regulated by extrinsic and intrinsic factors, with focus on maintenance of organismal homeostasis. The clue to change in physiology or metabolism of an organism, at times, can be derived from the changes in phenotypes. In the Drosophila melanogaster model system, GAL 4-overexpressed RNAi driver males (Mini-White Marker), targeted against specific genes, when crossed with Leon mutant (19-2/TM6B) females, yield progeny of different wing types. Different RNAi lines expressing the phenotypes in a gradient of sodden, mid to normal, explains the varying severity of the wing phenotypes. The comparison of flies co-expressed RNAi and Leon mutant with wild type or Leon mutant females, show changes in wing phenotype; in terms of wing venation, anterior cortical vein (acv) position, posterior cortical vein (pcv) position, bristles on the wing margins and the intersegmental distance. There is a distinct evidence of both rescue and deterioration phenotype observed at various levels, with the varying levels of RNAi expression in sodden, mid and normal type. A correlational study of these modified wing phenotypes to the physiological and metabolic functionalities, reveals the expression of most of these genes targeted by RNAi, mainly in the brain, heart, thoracic-abdominal ganglion, salivary gland, ovary and testis. Therefore, it can be hypothesized that the Leon mutant can be correlated with the RNAi.

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