alexa RNAi-Mediated Downregulation of Cyano-Glycoside Biosynt
ISSN: 2329-9029

Journal of Plant Biochemistry & Physiology
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Research Article

RNAi-Mediated Downregulation of Cyano-Glycoside Biosynthesis in Kenyan Cassava (Manihot esculenta Crantz) Genotypes

Piero NM1*, Murugi NJ2, Okoth OR1, Ombori OR3, Jalemba MA1 and Chelule CR2
1Department of Biochemistry and Biotechnology, Kenyatta University, P.O Box 437844-00100, Nairobi, Kenya
2Department of Environmental and Population Health, Kenyatta University, P.O Box 437844-00100, Nairobi, Kenya
3Department of Plant, Kenyatta University, P.O Box 437844-00100, Nairobi, Kenya
Corresponding Author : Piero NM
Department of Biochemistry and Biotechnology
Kenyatta University, P.OBox: 437844-00100
Nairobi, Kenya
Tel: +254710349485/+254732264557
E-mail: [email protected][email protected]
Received June 15, 2015; Accepted June 25, 2015; Published July 03, 2015
Citation: Piero NM, Murugi NJ, Okoth OR, Ombori OR, Jalemba MA, et al(2015) RNAi-Mediated Downregulation of Cyano-Glycoside Biosynthesis in Kenyan Cassava (Manihot esculenta Crantz) Genotypes. J Plant Biochem Physiol 3:148. doi:10.4172/2329-9029.1000148
Copyright: © 2015 Piero NM, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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Abstract

Cassava (Manihot esculenta Crantz) is an important perennial tropical crop for millions of people globally, particularly in subSaharan Africa. It is preferred due to its agronomical attributes such as ability to grow in poor soils and drought resistance. Cassava storage roots are a good source of starch. Further, cassava leaves and shoots are eaten as vegetables and are an excellent source of vitamins, minerals and protein. Cassava is also used in the production of ethanol for fuel, animal feed, and as a raw material for the starch industry. Cassava has high photosynthetic rates and its roots can persist in the soil for 8-24 months without decaying, thereby making it an ideal food security crop. In Kenya, cassava is a major source of subsistence and cash income to farmers in agroclimatically-disadvantaged regions and high potential areas of coast, Central and Western regions of Kenya. Sadly, cassava leaves and roots contain potentially toxic levels of cyanogenic glycosides. Cassava is largely propagated clonally making it an ideal plant for improvement through genetic engineering. This study was designed to produce transgenic acyanogenic cassava plants in which the expression of the cytochrome P450 genes (CYP79D1/D2) is downregulated through RNAi. Three Kenyan cassava genotypes; Adhiambo lera, Kibanda meno and Serere along with an exotic model cultivar TMS 60444 were used. The transformable lines were then taken through Agrobacterium-mediated transformation with an RNAi cassette harbouring cytochrome P450 genes (CYP92D1) to down regulate production of cyanoglycosides. Molecular analysis by PCR and RT-PCR confirmed transformation of the putative transformants.

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