alexa RNA-Seq Accuracy Comprehensive Assessment, Duplicability and Knowledge Content by the Sequencing Internal Control Consortium | OMICS International | Abstract
ISSN: 2157-7552

Journal of Tissue Science & Engineering
Open Access

Like us on:

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Review Article

RNA-Seq Accuracy Comprehensive Assessment, Duplicability and Knowledge Content by the Sequencing Internal Control Consortium

Leming Shi*

Bioinformatics Research Group, Boku University Vienna, Vienna, Austria

Corresponding Author:
Leming Shi
Bioinformatics Research Group
Boku University Vienna, Vienna, Austria
Tel: +1-870-543-7387
Email: [email protected]

Received date: September 15, 2014; Accepted date: December 23, 2014; Published date: December 26, 2014

Citation: Shi L (2015) RNA-Seq Accuracy Comprehensive Assessment, Duplicability and Knowledge Content by the Sequencing Internal Control Consortium. J Tissue Sci Eng 6:146. doi:10.4172/2157-7552.1000146

Copyright: © 2015 Shi L. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

We gift primary results from the Sequencing Internal Control (SEQC) project, coordinated by the America Food and Drug Administration. Examining Illumina HiSeq, Life Technologies SOLiD and Roche 454 platforms at multiple laboratory sites victimization reference RNA samples with intrinsical controls, we have a tendency to assess RNA sequencing (RNA-seq) performance for junction discovery and differential expression identification and compare it to microarray and quantitative PCR (qPCR) information victimization complementary metrics. in the slightest degree sequencing depths, we have a tendency to discover unannotated exon-exon junctions, with >80% valid by qPCR. We discover that measurements of relative expression area unit correct and reproducible across sites and platforms if specific filters area unit used. In distinction, RNA-seq and microarrays don't offer correct absolute measurements, and gene-specific biases area unit determined for all examined platforms, together with qPCR. Activity performance depends on the platform and information analysis pipeline, and variation is giant for transcript-level identification. The whole SEQC information sets, comprising >100 billion reads (10Tb), offer distinctive resources for evaluating RNA-seq analyses for clinical and regulative settings.

Keywords

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2018-19
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

+1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2018 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
Leave Your Message 24x7