Role of Adipose-Derived Stem Cells in Restoring Ovarian Structure of Adult Albino Rats with Chemotherapy-Induced Ovarian Failure: A Histological and Immunohistochemical Study
|Faten Riad Omar1, Noha Mohamed Afifi Amin1*, Hala Ahmed Elsherif2 and Dina Hisham Mohamed3|
|1Professor of Histology, Faculty of Medicine, Cairo University, Cairo, Egypt|
|2Lecturer of Histology, Faculty of Medicine, Cairo University, Cairo, Egypt|
|3Demonstrator of Histology, Faculty of Medicine, Cairo University, Cairo, Egypt|
|*Corresponding Author :||Noha Mohamed Afifi Amin
Faculty of Medicine, Cairo University
7 El-Tebarsy street
Kasr-Al-Aini street, Cairo, Egypt
E-mail: [email protected]
|Received: January 19, 2016; Accepted: February 11, 2016; Published: February 13, 2016|
|Citation: Omar FR, Amin NMA, Elsherif HA, Mohamed DH (2016) Role of Adipose-Derived Stem Cells in Restoring Ovarian Structure of Adult Albino Rats with Chemotherapy-Induced Ovarian Failure: A Histological and Immunohistochemical Study. J Carcinog Mutagen 7:254. doi:10.4172/2157-2518.1000254|
|Copyright: © 2016 Omar FR, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.|
Background and objectives: Damage to the reproductive system is one of the most devastating effects of chemotherapeutic treatment. It is frequently associated with premature ovarian failure (POF). Therefore, this study was planned to evaluate the therapeutic effectiveness of Adipose-Derived Stem Cells (ADSCs) in a rat model of chemotherapy-induced ovarian failure.
Methods and results: This study was carried out on forty adult female albino rats. They were divided into: Group I Control Group (n=8) received a vehicle of phosphate buffered saline (PBS) solution. Group II in which ovarian failure (OF) was induced using combined cyclophosphamide/busulfan therapy and were sacrificed after one week (gIIa n=8) and after five weeks (gIIb n=8). Group III in which rats received ADSCs after chemotherapy and were sacrificed after one week (gIIIa n=8) and after four weeks (gIIIb n=8). Blood samples were analysed for serum estradiol, FSH & LH. Ovarian sections were subjected to H&E, Masson´s Trichrome and immunohistochemical staining for anti-PCNA antibody. The mean number of primordial follicles, mean area % of collagen fibers, mean area % of +ve immunoreactivity for PCNA were measured by histomorphometric studies and statistically compared. ADSCs proved to have a therapeutic potential in improving ovarian structure and function following chemotherapy, evidenced at both the morphological and laboratory level.
Conclusions: The greatest effect of ADSCs was achieved after the longer duration of therapy which lasted for four weeks.