alexa Role of Plasmid-Borne Genes in the Biodegradation of Polycyclic Aromatic Hydrocarbons (PAHs) by Consortium of Aerobic Heterotrophic Bacteria

Journal of Petroleum & Environmental Biotechnology
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

Role of Plasmid-Borne Genes in the Biodegradation of Polycyclic Aromatic Hydrocarbons (PAHs) by Consortium of Aerobic Heterotrophic Bacteria

Isiodu GG*, Stanley OH, Ezebuiro Victor and Okerentugba PO
Department of Microbiology, Faculty of Science, University of Port Harcourt, Choba, P.M.B 5323, Port Harcourt, Nigeria
*Corresponding Author : Isiodu Godspower Gloria
Department of Microbiology, Faculty of Science
University of Port Harcourt, Choba, P.M.B 5323
Port Harcourt, Nigeria
Tel: +2348030926873
Received: November 28, 2015 Accepted: January 10, 2016 Published: January 10, 2016
Citation: Isiodu GG, Stanley OH, Victor E, Okerentugba PO (2016) Role of Plasmid-Borne Genes in the Biodegradation of Polycyclic Aromatic Hydrocarbons (PAHs) by Consortium of Aerobic Heterotrophic Bacteria. J Pet Environ Biotechnol 7:264. doi:10.4172/2157-7463.1000264
Copyright: © 2016 Isiodu GG, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


Aim: Certain plasmids play an important role in adaptation of natural microbial populations to oil and other hydrocarbons. This research investigated the role of plasmid-borne genes in the biodegradation of polycyclic aromatic hydrocarbons (PAHs) by a consortium of aerobic heterotrophic bacteria (AHB).
Methods: Physicochemical and microbiological characteristics of the water sample were analyzed using standard methods. AHB with the capacity for biodegradation were isolated from hydrocarbon polluted water from Bodo Creek using Bushnell Haas Agar. The positive isolates were subjected to plasmid profiling and curing. Three treatments: cured, uncured and control were used in the study. Curing was achieved with acridine orange. The degradation experiment was carried out in a 10 L glass bioreactor and monitored for 56 days using GC-MS analysis.
Results: Seven (7) out of 19 AHB isolates were selected for the degradation study. The 7 isolates, G1, G3, G19, GA5, GB3, GD1 and G12 were classified as Shewanella haliotis G1, Shewanella sp. G3, Vibrio alginolyticus G19, Pseudomonas putida GA5, Bacillus cereus GB3, B. pumilus GD1 and Shewanella sp. G12, respectively based on the phylogenetic analysis of their 16S rRNA genes. The sequences were deposited at the GenBank under the accession numbers KT886070-KT886076. The day 0 PAHs for cured, uncured and control treatments was 61.83 mg/L. After 56 days, the PAHs decreased to 2.90, 1.87 and 57.65 mg/L for cured, uncured and control treatments, respectively; representing percentage degradation of 95.31, 96.98 and 6.76%. The respective derived PAH degradation models for cured, uncured and control treatments were 32.614e-0.047t, 30.09e-0.05t and -0.0769t + 61.656. There was no significant difference (p<0.05) between PAH degradation in cured and uncured treatments.
Conclusion: The study demonstrated that PAH degradation by the consortium used in the study was chromosomal and not plasmid-borne.


Share This Page

Additional Info

Loading Please wait..
Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

1-702-714-7001Extn: 9037

Business & Management Journals


1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

1-702-714-7001Extn: 9042

General Science

Andrea Jason

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

1-702-714-7001Extn: 9042

© 2008- 2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version