RP-HPLC Method for Simultaneous Determination of Captopril and Diuretics: Application in Pharmaceutical Dosage Forms and Human SerumNajma Sultana1, M. Saeed Arayne1 and Safila Naveed2*
- *Corresponding Author:
- Safila Naveed
Faculty of Pharmacy
Jinnah University of Women Karachi Pakistan
E-mail: [email protected]
Received date: May 30, 2011; Accepted date: July 13, 2011; Published date: July 14, 2011
Citation: Sultana N, Arayne MS, Naveed S (2011) RP-HPLC Method for Simultaneous Determination of Captopril and Diuretics: Application in Pharmaceutical Dosage Forms and Human Serum. J Chromatograph Separat Techniq 2:109. doi:10.4172/2157-7064.1000109
Copyright: © 2011 Sultana N, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
An isocratic reversed phase high-performance liquid chromatographic (RP-HPLC) method has been developed for the simultaneous determination of captopril and diuretics (furosemide and hydrochlorothiazide) in API, dosage formulations and human serum. Chromatographic separation was achieved on Purospher Start C18 (250 mm x 4.6 mm, 5 μm) and Hypersil ODS C18 (150×4.6mm, 5micron) columns using mobile phase, methanol: water (70:30 v/v) adjusted to pH 3.0 via phosphoric acid 85% having flow rate of 1.0 mL min -1 at ambient temperature with detector set at 225 nm. Calibration curves were linear over range of 5-25 μg mL -1 with a correlation coefficient ± 0.999. LOD and LOQ were in the ranges of 0.4-2.3 μg mL -1 . Intra and inter-run precision and accuracy results were 98.0 to 102%.